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3rp7

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[[Image:3rp7.jpg|left|200px]]
 
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==Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD and uric acid==
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The line below this paragraph, containing "STRUCTURE_3rp7", creates the "Structure Box" on the page.
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<StructureSection load='3rp7' size='340' side='right'caption='[[3rp7]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3rp7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RP7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=URC:URIC+ACID'>URC</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3rp7| PDB=3rp7 | SCENE= }}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rp6|3rp6]], [[3rp8|3rp8]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp7 OCA], [https://pdbe.org/3rp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rp7 RCSB], [https://www.ebi.ac.uk/pdbsum/3rp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HpxO is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5-hydroxyisourate and functions in a novel pathway for purine catabolism found in Klebsiella pneumoniae. We have determined the structures of HpxO with and without uric acid at 2.0 A and 2.2 A, respectively. We have also determined the structure of the R204Q mutant at 2.0 A resolution in the absence of uric acid. The mutant structure is very similar to wild type HpxO except for the conformation of Arg103, which interacts with FAD in the mutant but not in the wild type structure. Interestingly, the R204Q mutation results in the uncoupling of NADH oxidation from uric acid hydroxylation. This suggests that Arg204 facilitates the deprotonation of uric acid activating it for the oxygen transfer. Based on these data, a mechanism for this reaction is proposed consisting of a nucleophilic attack of the urate anion on the flavin hydroperoxide resulting in the formation of 5-hydroxyisourate.
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===Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD and uric acid===
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Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae.,Hicks KA, O'Leary SE, Begley TP, Ealick SE Biochemistry. 2012 Dec 21. PMID:23259842<ref>PMID:23259842</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3rp7" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3rp7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP7 OCA].
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*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
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[[Category: Klebsiella pneumoniae]]
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== References ==
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[[Category: Begley, T P.]]
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<references/>
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[[Category: Ealick, S E.]]
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__TOC__
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[[Category: Hicks, K A.]]
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</StructureSection>
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[[Category: Leary, S E.O.]]
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[[Category: Klep7]]
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[[Category: Large Structures]]
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[[Category: Begley, T P]]
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[[Category: Ealick, S E]]
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[[Category: Hicks, K A]]
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[[Category: Leary, S E.O]]
[[Category: Fad-binding protein]]
[[Category: Fad-binding protein]]
[[Category: Monooxygenase]]
[[Category: Monooxygenase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]

Current revision

Crystal Structure of Klebsiella pneumoniae HpxO complexed with FAD and uric acid

PDB ID 3rp7

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