This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3rsc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:23, 22 June 2022) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3rsc.png|left|200px]]
 
-
<!--
+
==Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP and calicheamicin T0 bound form==
-
The line below this paragraph, containing "STRUCTURE_3rsc", creates the "Structure Box" on the page.
+
<StructureSection load='3rsc' size='340' side='right'caption='[[3rsc]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3rsc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15837 Atcc 15837]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RSC FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C0T:CALICHEAMICIN+T0'>C0T</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
-
-->
+
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
{{STRUCTURE_3rsc| PDB=3rsc | SCENE= }}
+
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3iaa|3iaa]]</div></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">calG2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 ATCC 15837])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rsc OCA], [https://pdbe.org/3rsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rsc RCSB], [https://www.ebi.ac.uk/pdbsum/3rsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rsc ProSAT]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.
-
===Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP and calicheamicin T0 bound form===
+
Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3rsc" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_21987796}}, adds the Publication Abstract to the page
+
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 21987796 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_21987796}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Atcc 15837]]
-
[[3rsc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RSC OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Bingman, C A]]
-
==Reference==
+
[[Category: Structural genomic]]
-
<ref group="xtra">PMID:021987796</ref><references group="xtra"/>
+
[[Category: Chang, A]]
-
[[Category: Micromonospora echinospora]]
+
[[Category: Helmich, K E]]
-
[[Category: Bingman, C A.]]
+
[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
-
[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
+
[[Category: Phillips, G N]]
-
[[Category: Chang, A.]]
+
[[Category: Singh, S]]
-
[[Category: Helmich, K E.]]
+
[[Category: Thorson, J S]]
-
[[Category: Phillips, G N.]]
+
-
[[Category: Singh, S.]]
+
-
[[Category: Thorson, J S.]]
+
-
[[Category: Center for eukaryotic structural genomic]]
+
[[Category: Cesg]]
[[Category: Cesg]]
[[Category: Enediyne]]
[[Category: Enediyne]]
 +
[[Category: Enzyme discovery for natural product biosynthesis]]
[[Category: Glycosyltransferase]]
[[Category: Glycosyltransferase]]
-
[[Category: Protein structure initiative]]
+
[[Category: Natpro]]
-
[[Category: Psi-2]]
+
[[Category: PSI, Protein structure initiative]]
-
[[Category: Structural genomic]]
+
[[Category: Tdp]]
[[Category: Tdp]]
[[Category: Transferase-antibiotic complex]]
[[Category: Transferase-antibiotic complex]]

Current revision

Crystal Structure of CalG2, Calicheamicin Glycosyltransferase, TDP and calicheamicin T0 bound form

PDB ID 3rsc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rsc"
Personal tools