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1hk9
From Proteopedia
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'''CRYSTAL STRUCTURE OF THE HFQ PROTEIN FROM ESCHERICHIA COLI''' | '''CRYSTAL STRUCTURE OF THE HFQ PROTEIN FROM ESCHERICHIA COLI''' | ||
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[[Category: Sauter, C.]] | [[Category: Sauter, C.]] | ||
[[Category: Suck, D.]] | [[Category: Suck, D.]] | ||
| - | [[Category: | + | [[Category: Hfq]] |
| - | [[Category: | + | [[Category: Pleiotropic regulator]] |
| - | [[Category: | + | [[Category: Rna binding protein]] |
| - | [[Category: | + | [[Category: Rna chaperone]] |
| - | [[Category: | + | [[Category: Sm-like]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:56:13 2008'' | |
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Revision as of 15:56, 2 May 2008
CRYSTAL STRUCTURE OF THE HFQ PROTEIN FROM ESCHERICHIA COLI
Overview
The Hfq protein was discovered in Escherichia coli in the early seventies as a host factor for the Qbeta phage RNA replication. During the last decade, it was shown to be involved in many RNA processing events and remote sequence homology indicated a link to spliceosomal Sm proteins. We report the crystal structure of the E.coli Hfq protein showing that its monomer displays a characteristic Sm-fold and forms a homo-hexamer, in agreement with former biochemical data. Overall, the structure of the E.coli Hfq ring is similar to the one recently described for Staphylococcus aureus. This confirms that bacteria contain a hexameric Sm-like protein which is likely to be an ancient and less specialized form characterized by a relaxed RNA binding specificity. In addition, we identified an Hfq ortholog in the archaeon Methanococcus jannaschii which lacks a classical Sm/Lsm gene. Finally, a detailed structural comparison shows that the Sm-fold is remarkably well conserved in bacteria, Archaea and Eukarya, and represents a universal and modular building unit for oligomeric RNA binding proteins.
About this Structure
1HK9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli., Sauter C, Basquin J, Suck D, Nucleic Acids Res. 2003 Jul 15;31(14):4091-8. PMID:12853626 Page seeded by OCA on Fri May 2 18:56:13 2008
