1hmh
From Proteopedia
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'''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME''' | '''THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HMH OCA]. | |
==Reference== | ==Reference== | ||
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422] | Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7969422 7969422] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Flaherty, K M.]] | [[Category: Flaherty, K M.]] | ||
[[Category: McKay, D B.]] | [[Category: McKay, D B.]] | ||
[[Category: Pley, H W.]] | [[Category: Pley, H W.]] | ||
| - | [[Category: | + | [[Category: Dna-rna hammerhead ribozyme]] |
| - | [[Category: | + | [[Category: Loop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:00:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 16:00, 2 May 2008
THREE-DIMENSIONAL STRUCTURE OF A HAMMERHEAD RIBOZYME
Overview
The hammerhead ribozyme is a small catalytic RNA motif made up of three base-paired stems and a core of highly conserved, non-complementary nucleotides essential for catalysis. The X-ray crystallographic structure of a hammerhead RNA-DNA ribozyme-inhibitor complex at 2.6 A resolution reveals that the base-paired stems are A-form helices and that the core has two structural domains. The first domain is formed by the sequence 5'-CUGA following stem I and is a sharp turn identical to the uridine turn of transfer RNA, whereas the second is a non-Watson-Crick three-base-pair duplex with a divalent-ion binding site. The phosphodiester backbone of the DNA inhibitor strand is splayed out at the phosphate 5' to the cleavage site. The structure indicates that the ribozyme may destabilize a substrate strand in order to facilitate twisting of the substrate to allow cleavage of the scissile bond.
About this Structure
Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of a hammerhead ribozyme., Pley HW, Flaherty KM, McKay DB, Nature. 1994 Nov 3;372(6501):68-74. PMID:7969422 Page seeded by OCA on Fri May 2 19:00:55 2008
