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3ul4

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Revision as of 07:44, 20 July 2022

Crystal structure of Coh-OlpA(Cthe_3080)-Doc918(Cthe_0918) complex: A novel type I Cohesin-Dockerin complex from Clostridium thermocellum ATTC 27405

Structural highlights

3ul4 is a 2 chain structure with sequence from Acet2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Gene:	ancA, Cthe_3080 (ACET2), Cthe_0918 (ACET2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ANCA_CLOTH] Anchors the cellulosome to the cell surface by binding the duplicated segment that is present at the C-terminal end of CipA.

Publication Abstract from PubMed

Protein-protein interactions play a pivotal role in a large number of biological processes exemplified by the assembly of the cellulosome. Integration of cellulosomal components occurs through the binding of type I cohesin modules located in a non-catalytic molecular scaffold to type I dockerin modules located at the C terminus of cellulosomal enzymes. The majority of type I dockerins display internal symmetry reflected by the presence of two essentially identical cohesin-binding surfaces. Here we report the crystal structures of two novel Clostridium thermocellum type I cohesin-dockerin complexes (CohOlpC-Doc124A and CohOlpA-Doc918). The data revealed that the two dockerins, Doc918 and Doc124A, are unusual because they lack the structural symmetry required to support a dual binding mode. Thus, in both cases, cohesin recognition is dominated by residues located at positions 11, 12, and 19 of one of the dockerin binding surfaces. The alternative binding mode is not possible (Doc918) or highly limited (Doc124A) because residues that assume the critical interacting positions, when dockerins are reoriented by 180 degrees , make steric clashes with the cohesin. In common with a third dockerin (Doc258) that also presents a single binding mode, Doc124A directs the appended cellulase, Cel124A, to the surface of C. thermocellum and not to cellulosomes because it binds preferentially to type I cohesins located at the cell envelope. Although there are a few exceptions, such as Doc918 described here, these data suggest that there is considerable selective pressure for the evolution of a dual binding mode in type I dockerins that direct enzymes into cellulosomes.

Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode.,Bras JL, Alves VD, Carvalho AL, Najmudin S, Prates JA, Ferreira LM, Bolam DN, Romao MJ, Gilbert HJ, Fontes CM J Biol Chem. 2012 Dec 28;287(53):44394-405. doi: 10.1074/jbc.M112.407700. Epub, 2012 Nov 1. PMID:23118225^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bras JL, Alves VD, Carvalho AL, Najmudin S, Prates JA, Ferreira LM, Bolam DN, Romao MJ, Gilbert HJ, Fontes CM. Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode. J Biol Chem. 2012 Dec 28;287(53):44394-405. doi: 10.1074/jbc.M112.407700. Epub, 2012 Nov 1. PMID:23118225 doi:http://dx.doi.org/10.1074/jbc.M112.407700

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ul4"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Coh-OlpA(Cthe_3080)-Doc918(Cthe_0918) complex: A novel type I Cohesin-Dockerin complex from Clostridium thermocellum ATTC 27405==
-<StructureSection load='3ul4' size='340' side='right' caption='[[3ul4]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+<StructureSection load='3ul4' size='340' side='right'caption='[[3ul4]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ul4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloth Cloth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UL4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ul4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acet2 Acet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UL4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ancA, Cthe_3080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 CLOTH]), Cthe_0918 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 CLOTH])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ancA, Cthe_3080 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 ACET2]), Cthe_0918 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 ACET2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ul4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ul4 OCA], [http://pdbe.org/3ul4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ul4 RCSB], [http://www.ebi.ac.uk/pdbsum/3ul4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ul4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ul4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ul4 OCA], [https://pdbe.org/3ul4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ul4 RCSB], [https://www.ebi.ac.uk/pdbsum/3ul4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ul4 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ANCA_CLOTH ANCA_CLOTH]] Anchors the cellulosome to the cell surface by binding the duplicated segment that is present at the C-terminal end of CipA.
+[[https://www.uniprot.org/uniprot/ANCA_CLOTH ANCA_CLOTH]] Anchors the cellulosome to the cell surface by binding the duplicated segment that is present at the C-terminal end of CipA.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Cloth]]
+[[Category: Acet2]]
+[[Category: Large Structures]]
 [[Category: Alves, V D]]
 [[Category: Bras, J]]

3ul4

From Proteopedia

Revision as of 07:44, 20 July 2022

Crystal structure of Coh-OlpA(Cthe_3080)-Doc918(Cthe_0918) complex: A novel type I Cohesin-Dockerin complex from Clostridium thermocellum ATTC 27405

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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