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3v0b
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==3.9 angstrom crystal structure of BoNT/Ai in complex with NTNHA== | |
| + | <StructureSection load='3v0b' size='340' side='right'caption='[[3v0b]], [[Resolution|resolution]] 3.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3v0b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V0B FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bont/a, boNT/A, bonta ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896]), ant, ntnh ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v0b OCA], [https://pdbe.org/3v0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v0b RCSB], [https://www.ebi.ac.uk/pdbsum/3v0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v0b ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Botulinum neurotoxins (BoNTs) are highly poisonous substances that are also effective medicines. Accidental BoNT poisoning often occurs through ingestion of Clostridium botulinum-contaminated food. Here, we present the crystal structure of a BoNT in complex with a clostridial nontoxic nonhemagglutinin (NTNHA) protein at 2.7 angstroms. Biochemical and functional studies show that NTNHA provides large and multivalent binding interfaces to protect BoNT from gastrointestinal degradation. Moreover, the structure highlights key residues in BoNT that regulate complex assembly in a pH-dependent manner. Collectively, our findings define the molecular mechanisms by which NTNHA shields BoNT in the hostile gastrointestinal environment and releases it upon entry into the circulation. These results will assist in the design of small molecules for inhibiting oral BoNT intoxication and of delivery vehicles for oral administration of biologics. | ||
| - | + | Botulinum neurotoxin is shielded by NTNHA in an interlocked complex.,Gu S, Rumpel S, Zhou J, Strotmeier J, Bigalke H, Perry K, Shoemaker CB, Rummel A, Jin R Science. 2012 Feb 24;335(6071):977-81. PMID:22363010<ref>PMID:22363010</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3v0b" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus botulinus van ermengem 1896]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bigalke, H]] | ||
| + | [[Category: Gu, S]] | ||
| + | [[Category: Jin, R]] | ||
| + | [[Category: Perry, K]] | ||
| + | [[Category: Rummel, A]] | ||
| + | [[Category: Rumpel, S]] | ||
| + | [[Category: Shoemaker, C B]] | ||
| + | [[Category: Strotmeier, J]] | ||
| + | [[Category: Zhou, J]] | ||
| + | [[Category: Botulinum neurotoxin]] | ||
| + | [[Category: Toxin]] | ||
| + | [[Category: Vhh free inlocked complex]] | ||
Current revision
3.9 angstrom crystal structure of BoNT/Ai in complex with NTNHA
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