This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3va2
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | [[Image:3va2.jpg|left|200px]] | ||
| - | + | ==Crystal structure of human Interleukin-5 in complex with its alpha receptor== | |
| + | <StructureSection load='3va2' size='340' side='right'caption='[[3va2]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3va2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VA2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IL5 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), IL5RA, IL5R ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3va2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3va2 OCA], [https://pdbe.org/3va2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3va2 RCSB], [https://www.ebi.ac.uk/pdbsum/3va2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3va2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/IL5_HUMAN IL5_HUMAN]] Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. [[https://www.uniprot.org/uniprot/IL5RA_HUMAN IL5RA_HUMAN]] This is the receptor for interleukin-5. The alpha chain binds to IL5. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Interleukin-5 (IL-5), a major hematopoietin, stimulates eosinophil proliferation, migration, and activation, which have been implicated in the pathogenesis of allergic inflammatory diseases, such as asthma. The specific IL-5 receptor (IL-5R) consists of the IL-5 receptor alpha subunit (IL-5RA) and the common receptor beta subunit (betac). IL-5 binding to IL-5R on target cells induces rapid tyrosine phosphorylation and activation of various cellular proteins, including JAK1/JAK2 and STAT1/STAT5. Here, we report the crystal structure of dimeric IL-5 in complex with the IL-5RA extracellular domains. The structure revealed that IL-5RA sandwiches the IL-5 homodimer by three tandem domains, arranged in a "wrench-like" architecture. This association mode was confirmed for human cells expressing IL-5 and the full-length IL-5RA by applying expanded genetic code technology: protein photo-cross-linking experiments revealed that the two proteins interact with each other in vivo in the same manner as that in the crystal structure. Furthermore, a comparison with the previously reported, partial GM-CSF*GM-CSFRA*betac structure enabled us to propose complete structural models for the IL-5 and GM-CSF receptor complexes, and to identify the residues conferring the cytokine-specificities of IL-5RA and GM-CSFRA. | ||
| - | + | Structural basis of interleukin-5 dimer recognition by its alpha receptor.,Kusano S, Kukimoto-Niino M, Hino N, Ohsawa N, Ikutani M, Takaki S, Sakamoto K, Hara-Yokoyama M, Shirouzu M, Takatsu K, Yokoyama S Protein Sci. 2012 Jun;21(6):850-64. doi: 10.1002/pro.2072. Epub 2012 Apr 23. PMID:22528658<ref>PMID:22528658</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3va2" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | [[ | + | *[[Interleukin 3D structures|Interleukin 3D structures]] |
| - | + | *[[Interleukin receptor 3D structures|Interleukin receptor 3D structures]] | |
| - | == | + | == References == |
| - | < | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Kukimoto-Niino, M | + | </StructureSection> |
| - | [[Category: Kusano, S | + | [[Category: Human]] |
| - | [[Category: Shirouzu, M | + | [[Category: Large Structures]] |
| - | [[Category: Yokoyama, S | + | [[Category: Kukimoto-Niino, M]] |
| + | [[Category: Kusano, S]] | ||
| + | [[Category: Shirouzu, M]] | ||
| + | [[Category: Yokoyama, S]] | ||
[[Category: Asthma]] | [[Category: Asthma]] | ||
[[Category: B cell growth]] | [[Category: B cell growth]] | ||
Current revision
Crystal structure of human Interleukin-5 in complex with its alpha receptor
| |||||||||||
