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3wwg

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==Crystal structure of the N-glycan-deficient variant N448A of isopullulanase complexed with isopanose==
==Crystal structure of the N-glycan-deficient variant N448A of isopullulanase complexed with isopanose==
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<StructureSection load='3wwg' size='340' side='right' caption='[[3wwg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='3wwg' size='340' side='right'caption='[[3wwg]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3wwg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/A._niger A. niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WWG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3wwg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/A._niger A. niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WWG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1wmr|1wmr]], [[1x0c|1x0c]], [[2z8g|2z8g]]</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wmr|1wmr]], [[1x0c|1x0c]], [[2z8g|2z8g]]</div></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ipuA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ipuA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isopullulanase Isopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.57 3.2.1.57] </span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Isopullulanase Isopullulanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.57 3.2.1.57] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wwg OCA], [http://pdbe.org/3wwg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wwg RCSB], [http://www.ebi.ac.uk/pdbsum/3wwg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wwg ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wwg OCA], [https://pdbe.org/3wwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wwg RCSB], [https://www.ebi.ac.uk/pdbsum/3wwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wwg ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/IPUA_ASPNG IPUA_ASPNG]] Hydrolyzes pullulan, a linear polymer which is composed of maltotriose units with alpha-1,6 glucosidic linkages, to produce isopanose (Glca1-4Glca1-6Glc).
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[[https://www.uniprot.org/uniprot/IPUA_ASPNG IPUA_ASPNG]] Hydrolyzes pullulan, a linear polymer which is composed of maltotriose units with alpha-1,6 glucosidic linkages, to produce isopanose (Glca1-4Glca1-6Glc).
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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[[Category: A. niger]]
[[Category: A. niger]]
[[Category: Isopullulanase]]
[[Category: Isopullulanase]]
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[[Category: Large Structures]]
[[Category: Miyazaki, T]]
[[Category: Miyazaki, T]]
[[Category: Nishikawa, A]]
[[Category: Nishikawa, A]]

Revision as of 05:33, 10 August 2022

Crystal structure of the N-glycan-deficient variant N448A of isopullulanase complexed with isopanose

PDB ID 3wwg

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