This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3x22
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of Escherichia coli nitroreductase NfsB mutant N71S/F123A/F124W== | ==Crystal structure of Escherichia coli nitroreductase NfsB mutant N71S/F123A/F124W== | ||
| - | <StructureSection load='3x22' size='340' side='right' caption='[[3x22]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3x22' size='340' side='right'caption='[[3x22]], [[Resolution|resolution]] 2.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3x22]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3x22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3X22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3X22 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ds7|1ds7]], [[3x21|3x21]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ds7|1ds7]], [[3x21|3x21]]</div></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nfnB, dprA, nfsB, nfsI, ntr ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nfnB, dprA, nfsB, nfsI, ntr ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3x22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3x22 OCA], [https://pdbe.org/3x22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3x22 RCSB], [https://www.ebi.ac.uk/pdbsum/3x22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3x22 ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/NFNB_ECOLI NFNB_ECOLI]] Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.<ref>PMID:15684426</ref> |
==See Also== | ==See Also== | ||
| Line 21: | Line 21: | ||
[[Category: 6,7-dihydropteridine reductase]] | [[Category: 6,7-dihydropteridine reductase]] | ||
[[Category: Ecoli]] | [[Category: Ecoli]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Bai, J]] | [[Category: Bai, J]] | ||
[[Category: Yang, J]] | [[Category: Yang, J]] | ||
Revision as of 05:38, 10 August 2022
Crystal structure of Escherichia coli nitroreductase NfsB mutant N71S/F123A/F124W
| |||||||||||
