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4a7p
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea== | |
| + | <StructureSection load='4a7p' size='340' side='right'caption='[[4a7p]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4a7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"pseudomonas_elodea"_kang_et_al._1982 "pseudomonas elodea" kang et al. 1982]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A7P FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/UDP-glucose_6-dehydrogenase UDP-glucose 6-dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.22 1.1.1.22] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a7p OCA], [https://pdbe.org/4a7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a7p RCSB], [https://www.ebi.ac.uk/pdbsum/4a7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a7p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gellan gum, a commercial gelling agent produced by Sphingomonas elodea ATCC 31461, is a high-value microbial exopolysaccharide. UDP-glucose dehydrogenase (UGD; EC 1.1.1.22) is responsible for the NAD-dependent twofold oxidation of UDP-glucose to UDP-glucuronic acid, one of the key components for gellan biosynthesis. S. elodea ATCC 31461 UGD, termed UgdG, was cloned, expressed, purified and crystallized in native and SeMet-derivatized forms in hexagonal and tetragonal space groups, respectively; the crystals diffracted X-rays to 2.40 and 3.40 A resolution, respectively. Experimental phases were obtained for the tetragonal SeMet-derivatized crystal form by a single-wavelength anomalous dispersion experiment. This structure was successfully used as a molecular-replacement probe for the hexagonal crystal form of the native protein. | ||
| - | + | Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461.,Rocha J, Granja AT, Sa-Correia I, Fialho A, Frazao C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt, 1):69-72. Epub 2009 Dec 25. PMID:20057075<ref>PMID:20057075</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4a7p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Pseudomonas elodea kang et al. 1982]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: UDP-glucose 6-dehydrogenase]] | ||
| + | [[Category: Fialho, A M]] | ||
| + | [[Category: Frazao, C]] | ||
| + | [[Category: Granja, A T]] | ||
| + | [[Category: Rocha, J]] | ||
| + | [[Category: Sa-Correia, I]] | ||
| + | [[Category: Carbohydrate synthesis]] | ||
| + | [[Category: Exopolysaccharide]] | ||
| + | [[Category: Gellan]] | ||
| + | [[Category: Oxidoreductase]] | ||
Current revision
Se-Met derivatized UgdG, UDP-glucose dehydrogenase from Sphingomonas elodea
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