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4agi
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4agi is ON HOLD Authors: Houser, J., Komarek, J., Kostlanova, N., Lahmann, M., Cioci, G., Varrot, A., Imberty, A., Wimmerova, M. Description: Cryst...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.== | |
| + | <StructureSection load='4agi' size='340' side='right'caption='[[4agi]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4agi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AGI FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SFU:METHYL+6-DEOXY-1-SELENO-ALPHA-L-GALACTOPYRANOSIDE'>SFU</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSK:S-(METHYLSELANYL)-L-CYSTEINE'>CSK</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4agt|4agt]], [[4ah4|4ah4]], [[4ah5|4ah5]], [[4aha|4aha]]</div></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4agi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4agi OCA], [https://pdbe.org/4agi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4agi RCSB], [https://www.ebi.ac.uk/pdbsum/4agi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4agi ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Aspergillus fumigatus is an important allergen and opportunistic pathogen. Similarly to many other pathogens, it is able to produce lectins that may be involved in the host-pathogen interaction. We focused on the lectin AFL, which was prepared in recombinant form and characterized. Its binding properties were studied using hemagglutination and glycan array analysis. We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including alpha1-6 linked core-fucose, which is an important marker for cancerogenesis. Other biologically relevant saccharides such as sialic acid, d-mannose or d-galactose were not bound. Blood group epitopes of the ABH and Lewis systems were recognized, Le(Y) being the preferred ligand among others. To provide a correlation between the observed functional characteristics and structural basis, AFL was crystallized in a complex with methyl-alpha,l-selenofucoside and its structure was solved using the SAD method. Six binding sites, each with different compositions, were identified per monomer and significant differences from the homologous AAL lectin were found. Structure-derived peptides were utilized to prepare anti-AFL polyclonal antibodies, which suggested the presence of AFL on the Aspergillus' conidia, confirming its expression in vivo. Stimulation of human bronchial cells by AFL led to IL-8 production in a dose-dependent manner. AFL thus probably contributes to the inflammatory response observed upon the exposure of a patient to A. fumigatus. The combination of affinity to human epithelial epitopes, production by conidia and pro-inflammatory activity is remarkable and shows that AFL might be an important virulence factor involved in an early stage of A. fumigatus infection. | ||
| - | + | A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal Pathogenicity.,Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr SC, Lahmann M, Balloy V, Fahy JV, Chignard M, Imberty A, Wimmerova M PLoS One. 2013 Dec 10;8(12):e83077. doi: 10.1371/journal.pone.0083077. PMID:24340081<ref>PMID:24340081</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4agi" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Cioci, G]] | ||
| + | [[Category: Houser, J]] | ||
| + | [[Category: Imberty, A]] | ||
| + | [[Category: Komarek, J]] | ||
| + | [[Category: Kostlanova, N]] | ||
| + | [[Category: Lahmann, M]] | ||
| + | [[Category: Varrot, A]] | ||
| + | [[Category: Wimmerova, M]] | ||
| + | [[Category: Sugar binding protein]] | ||
| + | [[Category: Sugar-binding protein]] | ||
Current revision
Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.
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