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4bwo

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==The FedF adhesin from entrrotoxigenic Escherichia coli is a sulfate- binding lectin==
==The FedF adhesin from entrrotoxigenic Escherichia coli is a sulfate- binding lectin==
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<StructureSection load='4bwo' size='340' side='right' caption='[[4bwo]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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<StructureSection load='4bwo' size='340' side='right'caption='[[4bwo]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4bwo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BWO FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4bwo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BWO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4b4p|4b4p]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwo OCA], [https://pdbe.org/4bwo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bwo RCSB], [https://www.ebi.ac.uk/pdbsum/4bwo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwo ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwo OCA], [http://pdbe.org/4bwo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4bwo RCSB], [http://www.ebi.ac.uk/pdbsum/4bwo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4bwo ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/Q47212_ECOLX Q47212_ECOLX]]
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F18-positive enterotoxigenic and Shiga toxin-producing Escherichia coli are responsible for post-weaning diarrhoea and oedema disease in pigs and lead to severe production losses in the farming industry. F18 fimbriae attach to the small intestine of young piglets by latching onto glycosphingolipids with A/H blood group determinants on type 1 core. We demonstrate the N-terminal domain of the F18 fimbrial subunit FedF to be responsible for ABH-mediated attachment and present its X-ray structure in ligand-free form and bound to A and B type 1 hexaoses. The FedF lectin domain comprises a 10-stranded immunoglobulin-like beta-sandwich. Three linear motives, Q(47) -N(50) , H(88) -S(90) and R(117) -T(119) , form a shallow glycan binding pocket near the tip of the domain that is selective for type 1 core glycans in extended conformation. In addition to the glycan binding pocket, a polybasic loop on the membrane proximal surface of FedF lectin domain is shown to be required for binding to piglet enterocytes. Although dispensable for ABH glycan recognition, the polybasic surface adds binding affinity in the context of the host cell membrane, a mechanism that is proposed to direct ABH-glycan binding to cell-bound glycosphingolipids and could allow bacteria to avoid clearance by secreted glycoproteins.
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Structural insight in histo-blood group binding by the F18 fimbrial adhesin FedF.,Moonens K, Bouckaert J, Coddens A, Tran T, Panjikar S, De Kerpel M, Cox E, Remaut H, De Greve H Mol Microbiol. 2012 Jul 19. doi: 10.1111/j.1365-2958.2012.08174.x. PMID:22812428<ref>PMID:22812428</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4bwo" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Adhesin|Adhesin]]
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*[[Adhesin 3D structures|Adhesin 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
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[[Category: Boer, A R.de]]
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[[Category: Large Structures]]
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[[Category: Bouckaert, J]]
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[[Category: Bouckaert J]]
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[[Category: Buts, L]]
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[[Category: Buts L]]
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[[Category: Deelder, A M]]
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[[Category: Deelder AM]]
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[[Category: Fabre, E]]
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[[Category: Fabre E]]
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[[Category: Guerardel, Y]]
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[[Category: Guerardel Y]]
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[[Category: Lonardi, E]]
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[[Category: Lonardi E]]
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[[Category: Moonens, K]]
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[[Category: Moonens K]]
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[[Category: Olsson, J D.M]]
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[[Category: Olsson JDM]]
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[[Category: Oscarson, S]]
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[[Category: Oscarson S]]
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[[Category: Weiss, M S]]
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[[Category: Weiss MS]]
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[[Category: Wuhrer, M]]
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[[Category: Wuhrer M]]
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[[Category: Cell adhesion]]
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[[Category: De Boer AR]]
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[[Category: Enterotoxigenic]]
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[[Category: Glycan array]]
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Current revision

The FedF adhesin from entrrotoxigenic Escherichia coli is a sulfate- binding lectin

PDB ID 4bwo

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