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7stm
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==Chitin Synthase 2 from Candida albicans bound to UDP-GlcNAc== | ==Chitin Synthase 2 from Candida albicans bound to UDP-GlcNAc== | ||
| - | <StructureSection load='7stm' size='340' side='right'caption='[[7stm]]' scene=''> | + | <StructureSection load='7stm' size='340' side='right'caption='[[7stm]], [[Resolution|resolution]] 3.02Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7STM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7STM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7stm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7STM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7STM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7stm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7stm OCA], [https://pdbe.org/7stm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7stm RCSB], [https://www.ebi.ac.uk/pdbsum/7stm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7stm ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7stm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7stm OCA], [https://pdbe.org/7stm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7stm RCSB], [https://www.ebi.ac.uk/pdbsum/7stm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7stm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A1D8PTV3_CANAL A0A1D8PTV3_CANAL]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Chitin is an essential component of the fungal cell wall. Chitin synthases (Chss) catalyze chitin formation and translocation across the membrane and are targets of antifungal agents, including nikkomycin Z and polyoxin D. Lack of structural insights into the action of these inhibitors on Chs has hampered their further development to the clinic. We present the cryo-EM structures of Chs2 from Candida albicans (CaChs2) in the apo, substrate-bound, nikkomycin Z-bound, and polyoxin D-bound states. CaChs2 adopts a unique domain-swapped dimer configuration where a conserved motif in the domain-swapped region controls enzyme activity. CaChs2 has a dual regulation mechanism where the chitin translocation tunnel is closed by the extracellular gate and plugged by a lipid molecule in the apo state to prevent non-specific leak. Analyses of substrate and inhibitor binding provide insights into the chemical logic of Chs inhibition, which can guide Chs-targeted antifungal development. | ||
| + | |||
| + | Structural basis for inhibition and regulation of a chitin synthase from Candida albicans.,Ren Z, Chhetri A, Guan Z, Suo Y, Yokoyama K, Lee SY Nat Struct Mol Biol. 2022 Jul 4. pii: 10.1038/s41594-022-00791-x. doi:, 10.1038/s41594-022-00791-x. PMID:35788183<ref>PMID:35788183</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7stm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Candida albicans]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chhetri A]] | [[Category: Chhetri A]] | ||
Current revision
Chitin Synthase 2 from Candida albicans bound to UDP-GlcNAc
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