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7qd1

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<StructureSection load='7qd1' size='340' side='right'caption='[[7qd1]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
<StructureSection load='7qd1' size='340' side='right'caption='[[7qd1]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7qd1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7qd1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QD1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QD1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd1 OCA], [https://pdbe.org/7qd1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qd1 OCA], [https://pdbe.org/7qd1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qd1 RCSB], [https://www.ebi.ac.uk/pdbsum/7qd1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qd1 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/A0A1J1JHR9_PLAAG A0A1J1JHR9_PLAAG]]
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The orange carotenoid protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection. Here, we report on the functional, spectral and structural characteristics of the peculiar Planktothrix PCC7805 OCP (Plankto-OCP). We show that this OCP variant is characterized by higher photoactivation and recovery rates, and a stronger energy-quenching activity, compared to other OCP studied thus far. We characterize the effect of the functionalizing carotenoid and of his-tagging on these reactions, and identify the time scales on which these modifications affect photoactivation. The presence of a his-tag at the C-terminus has a large influence on photoactivation, thermal recovery and PBS-fluorescence quenching, and likewise for the nature of the carotenoid that additionally affects the yield and characteristics of excited states and the ns-s dynamics of photoactivated OCP. By solving the structures of Plankto-OCP in the ECN- and CAN-functionalized states, each in two closely-related crystal forms, we further unveil the molecular breathing motions that animate Plankto-OCP at the monomer and dimer levels. We finally discuss the structural changes that could explain the peculiar properties of Plankto-OCP.
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Structure-function-dynamics relationships in the peculiar Planktothrix PCC7805 OCP1: Impact of his-tagging and carotenoid type.,Wilson A, Andreeva EA, Nizinski SJ, Talbot L, Hartmann E, Schlichting I, Burdzinski G, Sliwa M, Kirilovsky D, Colletier JP Biochim Biophys Acta Bioenerg. 2022 Jun 23;1863(7):148584. doi:, 10.1016/j.bbabio.2022.148584. PMID:35752265<ref>PMID:35752265</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7qd1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Andreeva, E A]]
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[[Category: Planktothrix agardhii]]
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[[Category: Colletier, J P]]
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[[Category: Andreeva EA]]
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[[Category: Hartmann, E]]
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[[Category: Colletier J-P]]
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[[Category: Schlichting, I]]
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[[Category: Hartmann E]]
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[[Category: Carotenoid binding protein]]
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[[Category: Schlichting I]]
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[[Category: Carotenoid-binding]]
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[[Category: Photoprotection]]
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[[Category: Photosynthesis]]
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Revision as of 06:24, 8 September 2022

Structure of the orange carotenoid protein from Planktothrix agardhii binding echinenone in the P21 space group

PDB ID 7qd1

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