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7tti

From Proteopedia

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Current revision (06:26, 8 September 2022) (edit) (undo)
 
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<StructureSection load='7tti' size='340' side='right'caption='[[7tti]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
<StructureSection load='7tti' size='340' side='right'caption='[[7tti]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7tti]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TTI FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7tti]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TTI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JUX:N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-[(6-phenylpyridazin-3-yl)sulfanyl]acetamide'>JUX</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JUX:N-cyclopropyl-N-(4-methyl-1,3-thiazol-2-yl)-2-[(6-phenylpyridazin-3-yl)sulfanyl]acetamide'>JUX</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tti OCA], [https://pdbe.org/7tti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tti RCSB], [https://www.ebi.ac.uk/pdbsum/7tti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tti ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tti OCA], [https://pdbe.org/7tti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tti RCSB], [https://www.ebi.ac.uk/pdbsum/7tti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tti ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/S12A4_HUMAN S12A4_HUMAN]] Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity.
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[[https://www.uniprot.org/uniprot/S12A4_HUMAN S12A4_HUMAN]] Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cation-chloride cotransporters (CCCs) catalyze electroneutral symport of Cl(-) with Na(+) and/or K(+) across membranes. CCCs are fundamental in cell volume homeostasis, transepithelia ion movement, maintenance of intracellular Cl(-) concentration, and neuronal excitability. Here, we present a cryoelectron microscopy structure of human K(+)-Cl(-) cotransporter (KCC)1 bound with the VU0463271 inhibitor in an outward-open state. In contrast to many other amino acid-polyamine-organocation transporter cousins, our first outward-open CCC structure reveals that opening the KCC1 extracellular ion permeation path does not involve hinge-bending motions of the transmembrane (TM) 1 and TM6 half-helices. Instead, rocking of TM3 and TM8, together with displacements of TM4, TM9, and a conserved intracellular loop 1 helix, underlie alternate opening and closing of extracellular and cytoplasmic vestibules. We show that KCC1 intriguingly exists in one of two distinct dimeric states via different intersubunit interfaces. Our studies provide a blueprint for understanding the mechanisms of CCCs and their inhibition by small molecule compounds.
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Structure of the human cation-chloride cotransport KCC1 in an outward-open state.,Zhao Y, Shen J, Wang Q, Ruiz Munevar MJ, Vidossich P, De Vivo M, Zhou M, Cao E Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2109083119. doi:, 10.1073/pnas.2109083119. Epub 2022 Jun 27. PMID:35759661<ref>PMID:35759661</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7tti" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Cao, E H]]
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[[Category: Cao EH]]
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[[Category: Zhao, Y X]]
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[[Category: Zhao YX]]
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[[Category: Outward-open state]]
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[[Category: Slc12a4]]
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[[Category: Transport protein-inhibitor complex]]
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[[Category: Vu0463271]]
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Current revision

Human KCC1 bound with VU0463271 In an outward-open state

PDB ID 7tti

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