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7xsd
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of RuBisCO assembly intermediate RbcL8Raf18RbcX16== | |
| - | + | <StructureSection load='7xsd' size='340' side='right'caption='[[7xsd]], [[Resolution|resolution]] 3.30Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[7xsd]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XSD FirstGlance]. <br> | |
| - | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xsd OCA], [https://pdbe.org/7xsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xsd RCSB], [https://www.ebi.ac.uk/pdbsum/7xsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xsd ProSAT]</span></td></tr> | |
| - | [[Category: | + | </table> |
| - | [[Category: | + | == Function == |
| - | [[Category: | + | [[https://www.uniprot.org/uniprot/RBCX_NOSS1 RBCX_NOSS1]] An RbcL-specific chaperone. The central cleft of the RbcX homodimer (RbcX2) binds the C-terminus of an RbcL monomer, stabilizing the C-terminus and probably preventing its reassociation with chaperonin GroEL-ES. At the same time the peripheral region of RbcX2 binds a second RbcL monomer, bridging the RbcL homodimers in the correct orientation. The RbcX2(2)-bound RbcL dimers then assemble into the RbcL8 core (RbcL8-(RbcX2)8). RbcS binding triggers the release of RbcX2.[HAMAP-Rule:MF_00855] When rbcL-rbcX-rbcS or rbcL-rbcS were overexpressed in E.coli no change in reconstituted RuBisCO activity was observed, which suggests RbcX plays no role in RuBisCO assembly in this system (PubMed:8472962). However in PubMed:8472962 E.coli chaperones groL and groS were also overexpressed, which may compensate for lack of rbcX (Probable).<ref>PMID:8472962</ref> <ref>PMID:15564522</ref> |
| - | [[Category: Xia | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] | ||
| + | [[Category: Jiang YL]] | ||
| + | [[Category: Xia LY]] | ||
| + | [[Category: Zhou CZ]] | ||
Current revision
Cryo-EM structure of RuBisCO assembly intermediate RbcL8Raf18RbcX16
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