7zcv

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<StructureSection load='7zcv' size='340' side='right'caption='[[7zcv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='7zcv' size='340' side='right'caption='[[7zcv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7zcv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZCV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7zcv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZCV FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zcv OCA], [https://pdbe.org/7zcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zcv RCSB], [https://www.ebi.ac.uk/pdbsum/7zcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zcv ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zcv OCA], [https://pdbe.org/7zcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zcv RCSB], [https://www.ebi.ac.uk/pdbsum/7zcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zcv ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/A0A0E7KI02_STREE A0A0E7KI02_STREE]]
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The Streptococcus pneumoniae Rgg144/SHP144 regulator-peptide quorum sensing (QS) system is critical for nutrient utilization, oxidative stress response, and virulence. Here, we characterized this system by assessing the importance of each residue within the active short hydrophobic peptide (SHP) by alanine-scanning mutagenesis and testing the resulting peptides for receptor binding and activation of the receptor. Interestingly, several of the mutations had little effect on binding to Rgg144 but reduced transcriptional activation appreciably. In particular, a proline substitution (P21A) reduced transcriptional activation by 29-fold but bound with a 3-fold higher affinity than the wild-type SHP. Consistent with the function of Rgg144, the mutant peptide led to decreased utilization of mannose and increased susceptibility to superoxide generator paraquat. Pangenome comparison showed full conservation of P21 across SHP144 allelic variants. Crystallization of Rgg144 in the absence of peptide revealed a comparable structure to the DNA bound and free forms of its homologs suggesting similar mechanisms of activation. Together, these analyses identify key interactions in a critical pneumococcal QS system. Further manipulation of the SHP has the potential to facilitate the development of inhibitors that are functional across strains. The approach described here is likely to be effective across QS systems in multiple species.
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Structure-function analysis for the development of peptide inhibitors for a Gram-positive quorum sensing system.,Abdullah IT, Ulijasz AT, Girija UV, Tam S, Andrew P, Hiller NL, Wallis R, Yesilkaya H Mol Microbiol. 2022 May 16. doi: 10.1111/mmi.14921. PMID:35575437<ref>PMID:35575437</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7zcv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Girija, U V]]
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[[Category: Streptococcus pneumoniae]]
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[[Category: Wallis, R]]
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[[Category: Girija UV]]
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[[Category: Yesilkaya, H]]
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[[Category: Wallis R]]
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[[Category: Dna binding protein]]
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[[Category: Yesilkaya H]]
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[[Category: Quorum sensing protein]]
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[[Category: Transcription regulator]]
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Revision as of 07:26, 8 September 2022

Rgg144 of Streptococcus pneumoniae

PDB ID 7zcv

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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