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| | ==CRYSTAL STRUCTURE OF THE HAT domain of k. lactis RNA14== | | ==CRYSTAL STRUCTURE OF THE HAT domain of k. lactis RNA14== |
| - | <StructureSection load='4e6h' size='340' side='right' caption='[[4e6h]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4e6h' size='340' side='right'caption='[[4e6h]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4e6h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E6H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4e6h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E6H FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ooe|2ooe]], [[2ond|2ond]], [[4eba|4eba]], [[4e85|4e85]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6h OCA], [https://pdbe.org/4e6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e6h RCSB], [https://www.ebi.ac.uk/pdbsum/4e6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6h ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RNA14, KLLA0F26290g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e6h OCA], [http://pdbe.org/4e6h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4e6h RCSB], [http://www.ebi.ac.uk/pdbsum/4e6h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4e6h ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RNA14_KLULA RNA14_KLULA]] Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation (By similarity). | + | [[https://www.uniprot.org/uniprot/RNA14_KLULA RNA14_KLULA]] Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Candida sphaerica]] | + | [[Category: Kluyveromyces lactis NRRL Y-1140]] |
| - | [[Category: Paulson, A R]] | + | [[Category: Large Structures]] |
| - | [[Category: Tong, L]] | + | [[Category: Paulson AR]] |
| - | [[Category: Clp1]] | + | [[Category: Tong L]] |
| - | [[Category: Hat domain]]
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| - | [[Category: Heat repeat]]
| + | |
| - | [[Category: Pcf11]]
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| - | [[Category: Structural protein]]
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| Structural highlights
Function
[RNA14_KLULA] Component of the cleavage factor IA (CFIA) complex, which is involved in the endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation (By similarity).
Publication Abstract from PubMed
A large protein machinery is required for 3'-end processing of mRNA precursors in eukaryotes. Cleavage factor IA (CF IA), a complex in the 3'-end processing machinery in yeast, contains four subunits, Rna14, Rna15, Clp1, and Pcf11. Rna14 has a HAT (half a TPR) domain at the N terminus and a region at the C terminus that mediates interactions with Rna15. Rna15 contains a RNA recognition module (RRM) at the N terminus, followed by a hinge region. These two proteins are homologs of CstF-77 and CstF-64 in the cleavage stimulation factor (CstF) of the mammalian 3'-end processing machinery. We report the first crystal structure of Rna14 in complex with the hinge region of Rna15, and the structures of the HAT domain of Rna14 alone in two different crystal forms. The complex of the C-terminal region of Rna14 with the hinge region of Rna15 does not have strong interactions with the HAT domain of Rna14, and this complex is likely to function independently of the HAT domain. Like CstF-77, the HAT domain of Rna14 is also a tightly associated dimer with a highly elongated shape. However, there are large variations in the organization of this dimer among the Rna14 structures, and there are also significant structural differences to CstF-77. These observations suggest that the HAT domain and especially its dimer may have some inherent conformational variability.
Crystal structure of the Rna14-Rna15 complex.,Paulson AR, Tong L RNA. 2012 Apr 18. PMID:22513198[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Paulson AR, Tong L. Crystal structure of the Rna14-Rna15 complex. RNA. 2012 Apr 18. PMID:22513198 doi:10.1261/rna.032524.112
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