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4eiw

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Revision as of 07:13, 28 September 2022

Whole cytosolic region of atp-dependent metalloprotease FtsH (G399L)

Structural highlights

4eiw is a 6 chain structure with sequence from Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FTSH_THET8] Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins (By similarity).[HAMAP-Rule:MF_01458] Degrades preferentially unfolded substrates in a processive, ATP-dependent manner, usually after hydrophobic residues.[HAMAP-Rule:MF_01458]

Publication Abstract from PubMed

An ATP-dependent protease, FtsH, digests misassembled membrane proteins in order to maintain membrane integrity and digests short-lived soluble proteins in order to control their cellular regulation. This enzyme has an N-terminal transmembrane segment and a C-terminal cytosolic region consisting of an AAA+ ATPase domain and a protease domain. Here we present two crystal structures: the protease domain and the whole cytosolic region. The cytosolic region fully retains an ATP-dependent protease activity and adopts a three-fold-symmetric hexameric structure. The protease domains displayed a six-fold symmetry, while the AAA+ domains, each containing ADP, alternate two orientations relative to the protease domain, making "open" and "closed" interdomain contacts. Apparently, ATPase is active only in the closed form, and protease operates in the open form. The protease catalytic sites are accessible only through a tunnel following from the AAA+ domain of the adjacent subunit, raising a possibility of translocation of polypeptide substrate to the protease sites through this tunnel.

Structure of the whole cytosolic region of ATP-dependent protease FtsH.,Suno R, Niwa H, Tsuchiya D, Zhang X, Yoshida M, Morikawa K Mol Cell. 2006 Jun 9;22(5):575-85. PMID:16762831^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Suno R, Niwa H, Tsuchiya D, Zhang X, Yoshida M, Morikawa K. Structure of the whole cytosolic region of ATP-dependent protease FtsH. Mol Cell. 2006 Jun 9;22(5):575-85. PMID:16762831 doi:10.1016/j.molcel.2006.04.020

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4eiw"

@@ Line 1: / Line 1: @@
 ==Whole cytosolic region of atp-dependent metalloprotease FtsH (G399L)==
-<StructureSection load='4eiw' size='340' side='right' caption='[[4eiw]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
+<StructureSection load='4eiw' size='340' side='right'caption='[[4eiw]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4eiw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4eiw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ftsH, TTHA1492 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiw OCA], [https://pdbe.org/4eiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiw RCSB], [https://www.ebi.ac.uk/pdbsum/4eiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiw ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiw OCA], [http://pdbe.org/4eiw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4eiw RCSB], [http://www.ebi.ac.uk/pdbsum/4eiw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FTSH_THET8 FTSH_THET8]] Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins (By similarity).[HAMAP-Rule:MF_01458]  Degrades preferentially unfolded substrates in a processive, ATP-dependent manner, usually after hydrophobic residues.[HAMAP-Rule:MF_01458]
+[[https://www.uniprot.org/uniprot/FTSH_THET8 FTSH_THET8]] Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins (By similarity).[HAMAP-Rule:MF_01458]  Degrades preferentially unfolded substrates in a processive, ATP-dependent manner, usually after hydrophobic residues.[HAMAP-Rule:MF_01458]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Thet8]]
+[[Category: Large Structures]]
-[[Category: Morikawa, K]]
+[[Category: Thermus thermophilus HB8]]
-[[Category: Niwa, H]]
+[[Category: Morikawa K]]
-[[Category: Suno, R]]
+[[Category: Niwa H]]
-[[Category: Tsuchiya, D]]
+[[Category: Suno R]]
-[[Category: Yoshida, M]]
+[[Category: Tsuchiya D]]
-[[Category: Atpase]]
+[[Category: Yoshida M]]
-[[Category: Hydrolase]]
-[[Category: Walker motif]]

4eiw

From Proteopedia

Revision as of 07:13, 28 September 2022

Whole cytosolic region of atp-dependent metalloprotease FtsH (G399L)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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