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4etw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the Enzyme-ACP Substrate Gatekeeper Complex Required for Biotin Synthesis== | |
| + | <StructureSection load='4etw' size='340' side='right'caption='[[4etw]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4etw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri] and [https://en.wikipedia.org/wiki/Shigella_flexneri_5_str._8401 Shigella flexneri 5 str. 8401]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ETW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ETW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZMK:METHYL+7-{[2-({N-[(2S)-2-HYDROXY-3,3-DIMETHYL-4-(PHOSPHONOOXY)BUTANOYL]-BETA-ALANYL}AMINO)ETHYL]SULFANYL}-7-OXOHEPTANOATE'>ZMK</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4etw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4etw OCA], [https://pdbe.org/4etw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4etw RCSB], [https://www.ebi.ac.uk/pdbsum/4etw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4etw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/BIOH_SHIFL BIOH_SHIFL] The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.[HAMAP-Rule:MF_01260] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although the pimeloyl moiety was long known to be a biotin precursor, the mechanism of assembly of this C7 alpha,omega-dicarboxylic acid was only recently elucidated. In Escherichia coli, pimelate is made by bypassing the strict specificity of the fatty acid synthetic pathway. BioC methylates the free carboxyl of a malonyl thioester, which replaces the usual acetyl thioester primer. This atypical primer is transformed to pimeloyl-acyl carrier protein (ACP) methyl ester by two cycles of fatty acid synthesis. The question is, what stops this product from undergoing further elongation? Although BioH readily cleaves this product in vitro, the enzyme is nonspecific, which made assignment of its physiological substrate problematical, especially because another enzyme, BioF, could also perform this gatekeeping function. We report the 2.05-A resolution cocrystal structure of a complex of BioH with pimeloyl-ACP methyl ester and use the structure to demonstrate that BioH is the gatekeeper and its physiological substrate is pimeloyl-ACP methyl ester. | ||
| - | + | Structure of the enzyme-acyl carrier protein (ACP) substrate gatekeeper complex required for biotin synthesis.,Agarwal V, Lin S, Lukk T, Nair SK, Cronan JE Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17406-11. doi:, 10.1073/pnas.1207028109. Epub 2012 Oct 8. PMID:23045647<ref>PMID:23045647</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4etw" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]] | ||
| + | *[[Beta-ketoacyl carrier protein reductase 3D structures|Beta-ketoacyl carrier protein reductase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Shigella flexneri]] | ||
| + | [[Category: Shigella flexneri 5 str. 8401]] | ||
| + | [[Category: Agarwal V]] | ||
| + | [[Category: Nair SK]] | ||
Current revision
Structure of the Enzyme-ACP Substrate Gatekeeper Complex Required for Biotin Synthesis
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