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| | ==NO mechanism, slma== | | ==NO mechanism, slma== |
| - | <StructureSection load='4gfl' size='340' side='right' caption='[[4gfl]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='4gfl' size='340' side='right'caption='[[4gfl]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4gfl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep3 Klep3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GFL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GFL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4gfl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_342 Klebsiella pneumoniae 342]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GFL FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gck|4gck]], [[4gcl|4gcl]], [[4gct|4gct]], [[4gcu|4gcu]], [[4gfk|4gfk]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gfl OCA], [https://pdbe.org/4gfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gfl RCSB], [https://www.ebi.ac.uk/pdbsum/4gfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gfl ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slmA, KPK_0113 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=507522 KLEP3])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gfl OCA], [http://pdbe.org/4gfl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gfl RCSB], [http://www.ebi.ac.uk/pdbsum/4gfl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gfl ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SLMA_KLEP3 SLMA_KLEP3]] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions (By similarity). | + | [https://www.uniprot.org/uniprot/SLMA_KLEP3 SLMA_KLEP3] Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Klep3]] | + | [[Category: Klebsiella pneumoniae 342]] |
| - | [[Category: Schumacher, M A]] | + | [[Category: Large Structures]] |
| - | [[Category: Cell division]] | + | [[Category: Schumacher MA]] |
| - | [[Category: Cytokinesis]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: Ftsz]]
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| - | [[Category: No]]
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| - | [[Category: Nucleoid occlusion]]
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| - | [[Category: Slma]]
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| Structural highlights
Function
SLMA_KLEP3 Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions (By similarity).
Publication Abstract from PubMed
The spatial and temporal control of Filamenting temperature sensitive mutant Z (FtsZ) Z-ring formation is crucial for proper cell division in bacteria. In Escherichia coli, the synthetic lethal with a defective Min system (SlmA) protein helps mediate nucleoid occlusion, which prevents chromosome fragmentation by binding FtsZ and inhibiting Z-ring formation over the nucleoid. However, to perform its function, SlmA must be bound to the nucleoid. To deduce the basis for this chromosomal requirement, we performed biochemical, cellular, and structural studies. Strikingly, structures show that SlmA dramatically distorts DNA, allowing it to bind as an orientated dimer-of-dimers. Biochemical data indicate that SlmA dimer-of-dimers can spread along the DNA. Combined structural and biochemical data suggest that this DNA-activated SlmA oligomerization would prevent FtsZ protofilament propagation and bundling. Bioinformatic analyses localize SlmA DNA sites near membrane-tethered chromosomal regions, and cellular studies show that SlmA inhibits FtsZ reservoirs from forming membrane-tethered Z rings. Thus, our combined data indicate that SlmA DNA helps block Z-ring formation over chromosomal DNA by forming higher-order protein-nucleic acid complexes that disable FtsZ filaments from coalescing into proper structures needed for Z-ring creation.
SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.,Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA Proc Natl Acad Sci U S A. 2013 Jun 10. PMID:23754405[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA. SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid. Proc Natl Acad Sci U S A. 2013 Jun 10. PMID:23754405 doi:10.1073/pnas.1221036110
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