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| | ==Crystal structure of the full DNA binding domain of GATA3-complex 2== | | ==Crystal structure of the full DNA binding domain of GATA3-complex 2== |
| - | <StructureSection load='4hc7' size='340' side='right' caption='[[4hc7]], [[Resolution|resolution]] 2.65Å' scene=''> | + | <StructureSection load='4hc7' size='340' side='right'caption='[[4hc7]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hc7]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HC7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HC7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hc7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HC7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hc9|4hc9]], [[4hca|4hca]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hc7 OCA], [https://pdbe.org/4hc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hc7 RCSB], [https://www.ebi.ac.uk/pdbsum/4hc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hc7 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GATA3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hc7 OCA], [http://pdbe.org/4hc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hc7 RCSB], [http://www.ebi.ac.uk/pdbsum/4hc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hc7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/GATA3_HUMAN GATA3_HUMAN]] Precursor B-cell acute lymphoblastic leukemia;Hypoparathyroidism - deafness - renal disease. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/GATA3_HUMAN GATA3_HUMAN] Precursor B-cell acute lymphoblastic leukemia;Hypoparathyroidism - deafness - renal disease. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GATA3_HUMAN GATA3_HUMAN]] Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.<ref>PMID:23824597</ref> | + | [https://www.uniprot.org/uniprot/GATA3_HUMAN GATA3_HUMAN] Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.<ref>PMID:23824597</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Bates, D L]] | + | [[Category: Large Structures]] |
| - | [[Category: Chen, L]] | + | [[Category: Bates DL]] |
| - | [[Category: Chen, Y]] | + | [[Category: Chen L]] |
| - | [[Category: Dey, R]] | + | [[Category: Chen Y]] |
| - | [[Category: Gata transcription factor]] | + | [[Category: Dey R]] |
| - | [[Category: Transcription-dna complex]]
| + | |
| - | [[Category: Zinc finger]]
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| Structural highlights
Disease
GATA3_HUMAN Precursor B-cell acute lymphoblastic leukemia;Hypoparathyroidism - deafness - renal disease. The disease is caused by mutations affecting the gene represented in this entry.
Function
GATA3_HUMAN Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.[1]
Publication Abstract from PubMed
GATA transcription factors regulate transcription during development and differentiation by recognizing distinct GATA sites with a tandem of two conserved zinc fingers, and by mediating long-range DNA looping. However, the molecular basis of these processes is not well understood. Here, we determined three crystal structures of the full DNA-binding domain (DBD) of human GATA3 protein, which contains both zinc fingers, in complex with different DNA sites. In one structure, both zinc fingers wrap around a palindromic GATA site, cooperatively enhancing the binding affinity and kinetic stability. Strikingly, in the other two structures, the two fingers of GATA DBD bind GATA sites on different DNA molecules, thereby bridging two separate DNA fragments. This was confirmed in solution by an in-gel fluorescence resonance energy transfer analysis. These findings not only provide insights into the structure and function of GATA proteins but also shed light on the molecular basis of long-range gene regulation.
DNA Binding by GATA Transcription Factor Suggests Mechanisms of DNA Looping and Long-Range Gene Regulation.,Chen Y, Bates DL, Dey R, Chen PH, Machado AC, Laird-Offringa IA, Rohs R, Chen L Cell Rep. 2012 Nov 29;2(5):1197-206. doi: 10.1016/j.celrep.2012.10.012. Epub 2012, Nov 8. PMID:23142663[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sasaki T, Onodera A, Hosokawa H, Watanabe Y, Horiuchi S, Yamashita J, Tanaka H, Ogawa Y, Suzuki Y, Nakayama T. Genome-Wide Gene Expression Profiling Revealed a Critical Role for GATA3 in the Maintenance of the Th2 Cell Identity. PLoS One. 2013 Jun 18;8(6):e66468. Print 2013. PMID:23824597 doi:http://dx.doi.org/10.1371/journal.pone.0066468
- ↑ Chen Y, Bates DL, Dey R, Chen PH, Machado AC, Laird-Offringa IA, Rohs R, Chen L. DNA Binding by GATA Transcription Factor Suggests Mechanisms of DNA Looping and Long-Range Gene Regulation. Cell Rep. 2012 Nov 29;2(5):1197-206. doi: 10.1016/j.celrep.2012.10.012. Epub 2012, Nov 8. PMID:23142663 doi:http://dx.doi.org/10.1016/j.celrep.2012.10.012
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