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| | ==Hendra virus Phosphoprotein C terminal domain== | | ==Hendra virus Phosphoprotein C terminal domain== |
| - | <StructureSection load='4heo' size='340' side='right' caption='[[4heo]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='4heo' size='340' side='right'caption='[[4heo]], [[Resolution|resolution]] 1.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4heo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hendh Hendh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HEO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4heo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hendra_virus_horse/Australia/Hendra/1994 Hendra virus horse/Australia/Hendra/1994]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HEO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4heo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4heo OCA], [https://pdbe.org/4heo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4heo RCSB], [https://www.ebi.ac.uk/pdbsum/4heo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4heo ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P/V/C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=928303 HENDH])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4heo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4heo OCA], [http://pdbe.org/4heo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4heo RCSB], [http://www.ebi.ac.uk/pdbsum/4heo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4heo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PHOSP_HENDH PHOSP_HENDH]] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template (By similarity). | + | [https://www.uniprot.org/uniprot/PHOSP_HENDH PHOSP_HENDH] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Hendh]] | + | [[Category: Hendra virus horse/Australia/Hendra/1994]] |
| - | [[Category: Jamin, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Tarbouriech, N]] | + | [[Category: Jamin M]] |
| - | [[Category: Yabukarski, F]] | + | [[Category: Tarbouriech N]] |
| - | [[Category: Phosphoprotein]] | + | [[Category: Yabukarski F]] |
| - | [[Category: Viral polymerase cofactor]]
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| - | [[Category: Viral protein]]
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| Structural highlights
Function
PHOSP_HENDH Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template (By similarity).
Publication Abstract from PubMed
Hendra virus (HeV) is a recently emerged severe human pathogen that belongs to the Henipavirus genus within the Paramyxoviridae family. The HeV genome is encapsidated by the nucleoprotein (N) within a helical nucleocapsid. Recruitment of the viral polymerase onto the nucleocapsid template relies on the interaction between the C-terminal domain, NTAIL, of N and the C-terminal X domain, XD, of the polymerase co-factor phosphoprotein (P). Here, we provide an atomic resolution description of the intrinsically disordered NTAIL domain in its isolated state and in intact nucleocapsids using nuclear magnetic resonance (NMR) spectroscopy. Using electron microscopy, we show that HeV nucleocapsids form herringbone-like structures typical of paramyxoviruses. We also report the crystal structure of XD of P that consists of a three-helix bundle. We study the interaction between NTAIL and XD using NMR titration experiments and provide a detailed mapping of the reciprocal binding sites. We show that the interaction is accompanied by alpha-helical folding of the molecular recognition element of NTAIL upon binding to a hydrophobic patch on the surface of XD. Finally, using solution NMR, we investigate the interaction between intact nucleocapsids and XD. Our results indicate that monomeric XD binds to NTAIL without triggering an additional unwinding of the nucleocapsid template. The present results provide a structural description at the atomic level of the protein-protein interactions required for transcription and replication of HeV, and the first direct observation of the interaction between the X domain of P and intact nucleocapsids in Paramyxoviridae.
Atomic Resolution Description of the Interaction between the Nucleoprotein and Phosphoprotein of Hendra Virus.,Communie G, Habchi J, Yabukarski F, Blocquel D, Schneider R, Tarbouriech N, Papageorgiou N, Ruigrok RW, Jamin M, Jensen MR, Longhi S, Blackledge M PLoS Pathog. 2013 Sep;9(9):e1003631. Epub 2013 Sep 26. PMID:24086133[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Communie G, Habchi J, Yabukarski F, Blocquel D, Schneider R, Tarbouriech N, Papageorgiou N, Ruigrok RW, Jamin M, Jensen MR, Longhi S, Blackledge M. Atomic Resolution Description of the Interaction between the Nucleoprotein and Phosphoprotein of Hendra Virus. PLoS Pathog. 2013 Sep;9(9):e1003631. Epub 2013 Sep 26. PMID:24086133 doi:http://dx.doi.org/10.1371/journal.ppat.1003631
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