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| | ==Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501== | | ==Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501== |
| - | <StructureSection load='4hic' size='340' side='right' caption='[[4hic]], [[Resolution|resolution]] 3.00Å' scene=''> | + | <StructureSection load='4hic' size='340' side='right'caption='[[4hic]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hic]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HIC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HIC FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF11 from conjugative plasmid pIP501 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hic OCA], [https://pdbe.org/4hic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hic RCSB], [https://www.ebi.ac.uk/pdbsum/4hic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hic ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hic OCA], [http://pdbe.org/4hic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hic RCSB], [http://www.ebi.ac.uk/pdbsum/4hic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hic ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8L1C9_ENTFL Q8L1C9_ENTFL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]] | + | [[Category: Enterococcus faecalis]] |
| - | [[Category: Goessweiner-Mohr, N]] | + | [[Category: Large Structures]] |
| - | [[Category: Keller, W]] | + | [[Category: Goessweiner-Mohr N]] |
| - | [[Category: Anti-parallel beta-sheet]]
| + | [[Category: Keller W]] |
| - | [[Category: Gram-positive]] | + | |
| - | [[Category: Pip501]]
| + | |
| - | [[Category: Type-iv secretion]]
| + | |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
Q8L1C9_ENTFL
Publication Abstract from PubMed
Conjugative plasmid transfer presents a serious threat to human health as the most important means of spreading antibiotic resistance and virulence genes among bacteria. The required direct cell-cell contact is established by a multi-protein complex, the conjugative type IV secretion system (T4SS). The conjugative core complex spans the cellular envelope and serves as a channel for macromolecular secretion. T4SSs of Gram-negative (G-) origin have been studied in great detail. In contrast, T4SSs of Gram-positive (G+) bacteria have only received little attention thus far, despite the medical relevance of numerous G+ pathogens (e.g. enterococci, staphylococci and streptococci). This study provides structural information on the type IV secretion (T4S) protein TraK of the G+ broad host range Enterococcus conjugative plasmid pIP501. The crystal structure of the N-terminally truncated construct TraKDelta was determined to 3.0 A resolution and exhibits a novel fold. Immunolocalization demonstrated that the protein localizes to the cell wall facing towards the cell exterior, but does not exhibit surface accessibility. Circular dichroism, dynamic light scattering and size-exclusion chromatography confirmed the protein to be a monomer. With the exception of proteins from closely related T4SSs, no significant sequence or structural relatives were found. This observation marks the protein as a very exclusive, specialized member of the pIP501 T4SS.
The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold.,Goessweiner-Mohr N, Fercher C, Arends K, Birner-Gruenberger R, Laverde-Gomez D, Huebner J, Grohmann E, Keller W Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):1124-35. doi:, 10.1107/S1399004714001606. Epub 2014 Mar 21. PMID:24699656[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Goessweiner-Mohr N, Fercher C, Arends K, Birner-Gruenberger R, Laverde-Gomez D, Huebner J, Grohmann E, Keller W. The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold. Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):1124-35. doi:, 10.1107/S1399004714001606. Epub 2014 Mar 21. PMID:24699656 doi:http://dx.doi.org/10.1107/S1399004714001606
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