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| | ==Crystal Structure of p11/Annexin A2 Heterotetramer in Complex with SMARCA3 Peptide== | | ==Crystal Structure of p11/Annexin A2 Heterotetramer in Complex with SMARCA3 Peptide== |
| - | <StructureSection load='4hre' size='340' side='right' caption='[[4hre]], [[Resolution|resolution]] 2.79Å' scene=''> | + | <StructureSection load='4hre' size='340' side='right'caption='[[4hre]], [[Resolution|resolution]] 2.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4hre]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HRE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HRE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4hre]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HRE FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Anxa2, Anx2, Cal1h ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), S100A10, ANX2LG, CAL1L, CLP11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hre OCA], [https://pdbe.org/4hre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hre RCSB], [https://www.ebi.ac.uk/pdbsum/4hre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hre ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hre OCA], [http://pdbe.org/4hre PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hre RCSB], [http://www.ebi.ac.uk/pdbsum/4hre PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hre ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ANXA2_MOUSE ANXA2_MOUSE]] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. [[http://www.uniprot.org/uniprot/HLTF_HUMAN HLTF_HUMAN]] Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.<ref>PMID:10391891</ref> <ref>PMID:18316726</ref> <ref>PMID:18719106</ref> <ref>PMID:7876228</ref> <ref>PMID:8672239</ref> <ref>PMID:9126292</ref> [[http://www.uniprot.org/uniprot/S10AA_HUMAN S10AA_HUMAN]] Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase. | + | [https://www.uniprot.org/uniprot/ANXA2_MOUSE ANXA2_MOUSE] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Annexin|Annexin]] | + | *[[Annexin 3D structures|Annexin 3D structures]] |
| - | *[[S100 protein|S100 protein]] | + | *[[S100 proteins 3D structures|S100 proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Gao, P]] | + | [[Category: Mus musculus]] |
| - | [[Category: Patel, D J]] | + | [[Category: Gao P]] |
| - | [[Category: Calcium binding]] | + | [[Category: Patel DJ]] |
| - | [[Category: Calcium-binding protein]]
| + | |
| - | [[Category: Nucleus]]
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| Structural highlights
Function
ANXA2_MOUSE Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Publication Abstract from PubMed
p11, through unknown mechanisms, is required for behavioral and cellular responses to selective serotonin reuptake inhibitors (SSRIs). We show that SMARCA3, a chromatin-remodeling factor, is a target for the p11/annexin A2 heterotetrameric complex. Determination of the crystal structure indicates that SMARCA3 peptide binds to a hydrophobic pocket in the heterotetramer. Formation of this complex increases the DNA-binding affinity of SMARCA3 and its localization to the nuclear matrix fraction. In the dentate gyrus, both p11 and SMARCA3 are highly enriched in hilar mossy cells and basket cells. The SSRI fluoxetine induces expression of p11 in both cell types and increases the amount of the ternary complex of p11/annexin A2/SMARCA3. SSRI-induced neurogenesis and behavioral responses are abolished by constitutive knockout of SMARCA3. Our studies indicate a central role for a chromatin-remodeling factor in the SSRI/p11 signaling pathway and suggest an approach to the development of improved antidepressant therapies. PAPERCLIP:
SMARCA3, a Chromatin-Remodeling Factor, Is Required for p11-Dependent Antidepressant Action.,Oh YS, Gao P, Lee KW, Ceglia I, Seo JS, Zhang X, Ahn JH, Chait BT, Patel DJ, Kim Y, Greengard P Cell. 2013 Feb 14;152(4):831-43. doi: 10.1016/j.cell.2013.01.014. PMID:23415230[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Oh YS, Gao P, Lee KW, Ceglia I, Seo JS, Zhang X, Ahn JH, Chait BT, Patel DJ, Kim Y, Greengard P. SMARCA3, a Chromatin-Remodeling Factor, Is Required for p11-Dependent Antidepressant Action. Cell. 2013 Feb 14;152(4):831-43. doi: 10.1016/j.cell.2013.01.014. PMID:23415230 doi:http://dx.doi.org/10.1016/j.cell.2013.01.014
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