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7oae

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(New page: '''Unreleased structure''' The entry 7oae is ON HOLD Authors: Effantin, G. Description: Cryo-EM structure of the plectasin fibril (double strands) Category: Unreleased Structures [...)
Current revision (20:22, 16 November 2022) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 7oae is ON HOLD
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==Cryo-EM structure of the plectasin fibril (double strands)==
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<StructureSection load='7oae' size='340' side='right'caption='[[7oae]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7oae]] is a 42 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoplectania_nigrella Pseudoplectania nigrella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OAE FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oae OCA], [https://pdbe.org/7oae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oae RCSB], [https://www.ebi.ac.uk/pdbsum/7oae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oae ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DEFPL_PSENR DEFPL_PSENR] Antimicrobial peptide that potently acts against several species of Gram-positive bacteria (PubMed:16222292, PubMed:19472324). It selectively inhibits peptidoglycan biosynthesis through complex formation with the cell wall precursor lipid II (1:1 molar ratio) thus inhibiting cell wall synthesis (PubMed:20508130). It does not disrupt cell membranes (PubMed:20508130). Is especially active against numerous clinical isolates of S.pneumoniae, including all 90 different serotypes and isolates resistant to clinically used antibiotics (PubMed:16222292). In vitro, shows considerable selectivity for bacteria over mammalian cells (PubMed:16222292). The peptide synthesized in D-amino acids does not show antibacterial activity (PubMed:19472324). In vitro, acts on voltage-gated potassium channels by moderately inhibiting mammalian Kv1.3/KCNA3 (IC(50)=2.8 uM), and moderately inhibiting others potassium channels (PubMed:25568977).<ref>PMID:16222292</ref> <ref>PMID:25568977</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Self-assembly and fibril formation play important roles in protein behaviour. Amyloid fibril formation is well-studied due to its role in neurodegenerative diseases and characterized by refolding of the protein into predominantly beta-sheet form. However, much less is known about the assembly of proteins into other types of supramolecular structures. Using cryo-electron microscopy at a resolution of 1.97 A, we show that a triple-mutant of the anti-microbial peptide plectasin, PPI42, assembles into helical non-amyloid fibrils. The in vitro anti-microbial activity was determined and shown to be enhanced compared to the wildtype. Plectasin contains a cysteine-stabilised alpha-helix-beta-sheet structure, which remains intact upon fibril formation. Two protofilaments form a right-handed protein fibril. The fibril formation is reversible and follows sigmoidal kinetics with a pH- and concentration dependent equilibrium between soluble monomer and protein fibril. This high-resolution structure reveals that alpha/beta proteins can natively assemble into fibrils.
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Authors: Effantin, G.
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pH- and concentration-dependent supramolecular assembly of a fungal defensin plectasin variant into helical non-amyloid fibrils.,Pohl C, Effantin G, Kandiah E, Meier S, Zeng G, Streicher W, Segura DR, Mygind PH, Sandvang D, Nielsen LA, Peters GHJ, Schoehn G, Mueller-Dieckmann C, Noergaard A, Harris P Nat Commun. 2022 Jun 7;13(1):3162. doi: 10.1038/s41467-022-30462-w. PMID:35672293<ref>PMID:35672293</ref>
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Description: Cryo-EM structure of the plectasin fibril (double strands)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Effantin, G]]
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<div class="pdbe-citations 7oae" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudoplectania nigrella]]
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[[Category: Effantin G]]

Current revision

Cryo-EM structure of the plectasin fibril (double strands)

PDB ID 7oae

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