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| | ==Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A== | | ==Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A== |
| - | <StructureSection load='4jkn' size='340' side='right' caption='[[4jkn]], [[Resolution|resolution]] 1.54Å' scene=''> | + | <StructureSection load='4jkn' size='340' side='right'caption='[[4jkn]], [[Resolution|resolution]] 1.54Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4jkn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JKN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4jkn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JKN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3uge|3uge]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jkn OCA], [https://pdbe.org/4jkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jkn RCSB], [https://www.ebi.ac.uk/pdbsum/4jkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jkn ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">azu, azu PA4922, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arsenate_reductase_(azurin) Arsenate reductase (azurin)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.20.9.1 1.20.9.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jkn OCA], [http://pdbe.org/4jkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4jkn RCSB], [http://www.ebi.ac.uk/pdbsum/4jkn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4jkn ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase. | + | [https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Azurin|Azurin]] | + | *[[Azurin 3D structures|Azurin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseae]] | + | [[Category: Large Structures]] |
| - | [[Category: Berry, S B]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Bladholm, E L]] | + | [[Category: Berry SB]] |
| - | [[Category: Masters, F M]] | + | [[Category: Bladholm EL]] |
| - | [[Category: Panzner, M J]] | + | [[Category: Masters FM]] |
| - | [[Category: Zampino, A P]] | + | [[Category: Panzner MJ]] |
| - | [[Category: Ziegler, C J]] | + | [[Category: Zampino AP]] |
| - | [[Category: Electron transport]] | + | [[Category: Ziegler CJ]] |
| - | [[Category: Mercury metallation]]
| + | |
| Structural highlights
Function
AZUR_PSEAE Transfers electrons from cytochrome c551 to cytochrome oxidase.
Publication Abstract from PubMed
Mercury(II) metallation of Pseudomonas aeruginosa azurin has been characterized structurally and biochemically. The X-ray crystal structure at 1.5A of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings are further validated by NMR, Matrix-assisted laser desorption/ionization spectrometry (MALDI), and UV-visible spectroscopic methods indicating copper displacement from the wild-type protein. Bioinformatic analysis has identified homologous human protein domains computationally, and compared them to the structure of azurin, providing a model for human mercury interactions. Study of the mercury-azurin adduct, in combination with other known examples of protein-heavy metal interactions, could provide further insight into the chemical mechanisms of toxicological interactions, leading toward a global understanding of the biological speciation of toxic heavy metals.
Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity.,Zampino AP, Masters FM, Bladholm EL, Panzner MJ, Berry SM, Leeper TC, Ziegler CJ J Inorg Biochem. 2014 Dec;141:152-60. doi: 10.1016/j.jinorgbio.2014.09.003. Epub , 2014 Sep 16. PMID:25265377[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zampino AP, Masters FM, Bladholm EL, Panzner MJ, Berry SM, Leeper TC, Ziegler CJ. Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity. J Inorg Biochem. 2014 Dec;141:152-60. doi: 10.1016/j.jinorgbio.2014.09.003. Epub , 2014 Sep 16. PMID:25265377 doi:http://dx.doi.org/10.1016/j.jinorgbio.2014.09.003
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