|
|
| Line 1: |
Line 1: |
| | | | |
| | ==Crystal structure of EGFP-based Calcium Sensor CatchER complexed with Gd== | | ==Crystal structure of EGFP-based Calcium Sensor CatchER complexed with Gd== |
| - | <StructureSection load='4l12' size='340' side='right' caption='[[4l12]], [[Resolution|resolution]] 1.78Å' scene=''> | + | <StructureSection load='4l12' size='340' side='right'caption='[[4l12]], [[Resolution|resolution]] 1.78Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4l12]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L12 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L12 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4l12]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L12 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l12 OCA], [https://pdbe.org/4l12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l12 RCSB], [https://www.ebi.ac.uk/pdbsum/4l12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l12 ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4l13|4l13]], [[4l1i|4l1i]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l12 OCA], [http://pdbe.org/4l12 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4l12 RCSB], [http://www.ebi.ac.uk/pdbsum/4l12 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4l12 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. | + | [https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 25: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aeqvi]] | + | [[Category: Aequorea victoria]] |
| - | [[Category: Weber, I T]] | + | [[Category: Large Structures]] |
| - | [[Category: Zhang, Y]] | + | [[Category: Weber IT]] |
| - | [[Category: Calcium binding protein]] | + | [[Category: Zhang Y]] |
| - | [[Category: Calcium sensor]]
| + | |
| - | [[Category: Chromophore]]
| + | |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Gadolinium]]
| + | |
| - | [[Category: Luminescence]]
| + | |
| - | [[Category: Metal ion]]
| + | |
| Structural highlights
Function
GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Publication Abstract from PubMed
Calcium ions, which are important signaling molecules, can be detected in the endoplasmic reticulum by an engineered mutant of green fluorescent protein (GFP) designated CatchER with a fast off-rate. High resolution (1.78-1.20 A) crystal structures were analyzed for CatchER in the apo form and in complexes with calcium or gadolinium to probe the binding site for metal ions. While CatchER exhibits a 1:1 binding stoichiometry in solution, two positions were observed for each of the metal ions bound within the hand-like site formed by the carboxylate side chains of the mutated residues S147E, S202D, Q204E, F223E and T225E that may be responsible for its fast kinetic properties. Comparison of the structures of CatchER, wild-type GFP and enhanced GFP confirmed that different conformations of Thr203 and Glu222 are associated with the two forms of Tyr66 of the chromophore which are responsible for the absorbance wavelengths of the different proteins. Calcium binding to CatchER may shift the equilibrium for conformational population of the Glu222 side chain and lead to further changes in its optical properties.
Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics.,Zhang Y, Reddish F, Tang S, Zhuo Y, Wang YF, Yang JJ, Weber IT Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2309-19. doi:, 10.1107/S0907444913021306. Epub 2013 Nov 19. PMID:24311573[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang Y, Reddish F, Tang S, Zhuo Y, Wang YF, Yang JJ, Weber IT. Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics. Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2309-19. doi:, 10.1107/S0907444913021306. Epub 2013 Nov 19. PMID:24311573 doi:http://dx.doi.org/10.1107/S0907444913021306
|
