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7ot9
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of the AI-2 exporter family protein YdiK from E. coli== | |
| + | <StructureSection load='7ot9' size='340' side='right'caption='[[7ot9]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7ot9]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OT9 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ot9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ot9 OCA], [https://pdbe.org/7ot9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ot9 RCSB], [https://www.ebi.ac.uk/pdbsum/7ot9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ot9 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YDIK_ECOLI YDIK_ECOLI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteria utilize small extracellular molecules to communicate in order to collectively coordinate their behaviors in response to the population density. Autoinducer-2 (AI-2), a universal molecule for both intra- and inter-species communication, is involved in the regulation of biofilm formation, virulence, motility, chemotaxis, and antibiotic resistance. While many studies have been devoted to understanding the biosynthesis and sensing of AI-2, very little information is available on its export. The protein TqsA from Escherichia coli, which belongs to the AI-2 exporter superfamily, has been shown to export AI-2. Here, we report the cryogenic electron microscopic structures of two AI-2 exporters (TqsA and YdiK) from E. coli at 3.35 A and 2.80 A resolutions, respectively. Our structures suggest that the AI-2 exporter exists as a homo-pentameric complex. In silico molecular docking and native mass spectrometry experiments were employed to demonstrate the interaction between AI-2 and TqsA, and the results highlight the functional importance of two helical hairpins in substrate binding. We propose that each monomer works as an independent functional unit utilizing an elevator-type transport mechanism. | ||
| - | + | Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.,Khera R, Mehdipour AR, Bolla JR, Kahnt J, Welsch S, Ermler U, Muenke C, Robinson CV, Hummer G, Xie H, Michel H EMBO J. 2022 Jun 14:e109990. doi: 10.15252/embj.2021109990. PMID:35698912<ref>PMID:35698912</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7ot9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli K-12]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Khera R]] | ||
| + | [[Category: Michel H]] | ||
| + | [[Category: Xie H]] | ||
Current revision
Structure of the AI-2 exporter family protein YdiK from E. coli
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