1n4x
From Proteopedia
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| - | [[Image:1n4x.jpg|left|200px]]<br /><applet load="1n4x" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1n4x, resolution 1.7Å" /> | ||
| - | '''Structure of scFv 1696 at acidic pH'''<br /> | ||
| - | == | + | ==Structure of scFv 1696 at acidic pH== |
| - | The monoclonal antibody 1696, which was raised against the HIV-1 protease, inhibits the catalytic activity of the enzyme from both the HIV-1 and | + | <StructureSection load='1n4x' size='340' side='right'caption='[[1n4x]], [[Resolution|resolution]] 1.70Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1n4x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4X FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4x OCA], [https://pdbe.org/1n4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4x RCSB], [https://www.ebi.ac.uk/pdbsum/1n4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/KV2A7_MOUSE KV2A7_MOUSE] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4x_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4x ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The monoclonal antibody 1696, which was raised against the HIV-1 protease, inhibits the catalytic activity of the enzyme from both the HIV-1 and HIV-2 strains. The antibody cross-reacts with peptides containing the N-terminus of the enzyme, which is highly conserved between these strains. The crystal structure of a single-chain Fv fragment of 1696 (scFv-1696) in the non-complexed form, solved at 1.7 A resolution, is compared with the previously reported non-complexed Fab-1696 and antigen-bound scFv-1696 structures. Large conformational changes in the third hypervariable region of the heavy chain and differences in relative orientation of the variable domains are observed between the different structures. | ||
| - | + | Structure of a single-chain Fv fragment of an antibody that inhibits the HIV-1 and HIV-2 proteases.,Lescar J, Brynda J, Fabry M, Horejsi M, Rezacova P, Sedlacek J, Bentley GA Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):955-7. Epub 2003, Apr 25. PMID:12777823<ref>PMID:12777823</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1n4x" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Bentley GA]] | ||
| + | [[Category: Brynda J]] | ||
| + | [[Category: Fabry M]] | ||
| + | [[Category: Horejsi M]] | ||
| + | [[Category: Lescar J]] | ||
| + | [[Category: Rezacova P]] | ||
| + | [[Category: Sedlacek J]] | ||
Current revision
Structure of scFv 1696 at acidic pH
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Categories: Large Structures | Mus musculus | Bentley GA | Brynda J | Fabry M | Horejsi M | Lescar J | Rezacova P | Sedlacek J
