1n4x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:47, 21 December 2022) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1n4x.jpg|left|200px]]
 
-
{{Structure
+
==Structure of scFv 1696 at acidic pH==
-
|PDB= 1n4x |SIZE=350|CAPTION= <scene name='initialview01'>1n4x</scene>, resolution 1.7&Aring;
+
<StructureSection load='1n4x' size='340' side='right'caption='[[1n4x]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
+
<table><tr><td colspan='2'>[[1n4x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N4X FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-
|GENE=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4x OCA], [https://pdbe.org/1n4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n4x RCSB], [https://www.ebi.ac.uk/pdbsum/1n4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n4x ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=[[1cl7|1cl7]], [[1jp5|1jp5]]
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4x OCA], [http://www.ebi.ac.uk/pdbsum/1n4x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n4x RCSB]</span>
+
[https://www.uniprot.org/uniprot/KV2A7_MOUSE KV2A7_MOUSE]
-
}}
+
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n4/1n4x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n4x ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The monoclonal antibody 1696, which was raised against the HIV-1 protease, inhibits the catalytic activity of the enzyme from both the HIV-1 and HIV-2 strains. The antibody cross-reacts with peptides containing the N-terminus of the enzyme, which is highly conserved between these strains. The crystal structure of a single-chain Fv fragment of 1696 (scFv-1696) in the non-complexed form, solved at 1.7 A resolution, is compared with the previously reported non-complexed Fab-1696 and antigen-bound scFv-1696 structures. Large conformational changes in the third hypervariable region of the heavy chain and differences in relative orientation of the variable domains are observed between the different structures.
-
'''Structure of scFv 1696 at acidic pH'''
+
Structure of a single-chain Fv fragment of an antibody that inhibits the HIV-1 and HIV-2 proteases.,Lescar J, Brynda J, Fabry M, Horejsi M, Rezacova P, Sedlacek J, Bentley GA Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):955-7. Epub 2003, Apr 25. PMID:12777823<ref>PMID:12777823</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1n4x" style="background-color:#fffaf0;"></div>
-
==Overview==
+
==See Also==
-
The monoclonal antibody 1696, which was raised against the HIV-1 protease, inhibits the catalytic activity of the enzyme from both the HIV-1 and HIV-2 strains. The antibody cross-reacts with peptides containing the N-terminus of the enzyme, which is highly conserved between these strains. The crystal structure of a single-chain Fv fragment of 1696 (scFv-1696) in the non-complexed form, solved at 1.7 A resolution, is compared with the previously reported non-complexed Fab-1696 and antigen-bound scFv-1696 structures. Large conformational changes in the third hypervariable region of the heavy chain and differences in relative orientation of the variable domains are observed between the different structures.
+
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
-
 
+
== References ==
-
==About this Structure==
+
<references/>
-
1N4X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N4X OCA].
+
__TOC__
-
 
+
</StructureSection>
-
==Reference==
+
[[Category: Large Structures]]
-
Structure of a single-chain Fv fragment of an antibody that inhibits the HIV-1 and HIV-2 proteases., Lescar J, Brynda J, Fabry M, Horejsi M, Rezacova P, Sedlacek J, Bentley GA, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):955-7. Epub 2003, Apr 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12777823 12777823]
+
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Protein complex]]
+
[[Category: Bentley GA]]
-
[[Category: Bentley, G A.]]
+
[[Category: Brynda J]]
-
[[Category: Brynda, J.]]
+
[[Category: Fabry M]]
-
[[Category: Fabry, M.]]
+
[[Category: Horejsi M]]
-
[[Category: Horejsi, M.]]
+
[[Category: Lescar J]]
-
[[Category: Lescar, J.]]
+
[[Category: Rezacova P]]
-
[[Category: Rezacova, P.]]
+
[[Category: Sedlacek J]]
-
[[Category: Sedlacek, J.]]
+
-
[[Category: immunoglobulin]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:29 2008''
+

Current revision

Structure of scFv 1696 at acidic pH

PDB ID 1n4x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n4x"
Personal tools