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4m33

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Revision as of 10:49, 21 December 2022

Crystal structure of gated-pore mutant H141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis

Structural highlights

4m33 is a 4 chain structure with sequence from Mycolicibacterium smegmatis MC2 155. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0QXB7_MYCS2

Publication Abstract from PubMed

Dps (DNA-binding protein from starved cells) are dodecameric assemblies belonging to the ferritin family that can bind DNA, carry out ferroxidation, and store iron in their shells. The ferritin-like trimeric pore harbors the channel for the entry and exit of iron. By representing the structure of Dps as a network we have identified a charge-driven interface formed by a histidine aspartate cluster at the pore interface unique to Mycobacterium smegmatis Dps protein, MsDps2. Site-directed mutagenesis was employed to generate mutants to disrupt the charged interactions. Kinetics of iron uptake/release of the wild type and mutants were compared. Crystal structures were solved at a resolution of 1.8-2.2 A for the various mutants to compare structural alterations vis a vis the wild type protein. The substitutions at the pore interface resulted in alterations in the side chain conformations leading to an overall weakening of the interface network, especially in cases of substitutions that alter the charge at the pore interface. Contrary to earlier findings where conserved aspartate residues were found crucial for iron release, we propose here that in the case of MsDps2, it is the interplay of negative-positive potentials at the pore that enables proper functioning of the protein. In similar studies in ferritins, negative and positive patches near the iron exit pore were found to be important in iron uptake/release kinetics. The unique ionic cluster in MsDps2 makes it a suitable candidate to act as nano-delivery vehicle, as these gated pores can be manipulated to exhibit conformations allowing for slow or fast rates of iron release.

A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis.,Williams SM, Chandran AV, Vijayabaskar MS, Roy S, Balaram H, Vishveshwara S, Vijayan M, Chatterji D J Biol Chem. 2014 Apr 18;289(16):11042-58. doi: 10.1074/jbc.M113.524421. Epub, 2014 Feb 26. PMID:24573673^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Williams SM, Chandran AV, Vijayabaskar MS, Roy S, Balaram H, Vishveshwara S, Vijayan M, Chatterji D. A histidine aspartate ionic lock gates the iron passage in miniferritins from Mycobacterium smegmatis. J Biol Chem. 2014 Apr 18;289(16):11042-58. doi: 10.1074/jbc.M113.524421. Epub, 2014 Feb 26. PMID:24573673 doi:http://dx.doi.org/10.1074/jbc.M113.524421

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4m33"

@@ Line 1: / Line 1: @@
 ==Crystal structure of gated-pore mutant H141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis==
-<StructureSection load='4m33' size='340' side='right' caption='[[4m33]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
+<StructureSection load='4m33' size='340' side='right'caption='[[4m33]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4m33]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M33 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M33 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4m33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M33 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4m32|4m32]], [[4m34|4m34]], [[4m35|4m35]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m33 OCA], [https://pdbe.org/4m33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m33 RCSB], [https://www.ebi.ac.uk/pdbsum/4m33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m33 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DPS2, MSMEG_3242, MSMEI_3159 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m33 OCA], [http://pdbe.org/4m33 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m33 RCSB], [http://www.ebi.ac.uk/pdbsum/4m33 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m33 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0QXB7_MYCS2 A0QXB7_MYCS2]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Mycs2]]
+[[Category: Large Structures]]
-[[Category: Balaram, H]]
+[[Category: Mycolicibacterium smegmatis MC2 155]]
-[[Category: Chandran, A V]]
+[[Category: Balaram H]]
-[[Category: Chatterji, D]]
+[[Category: Chandran AV]]
-[[Category: Roy, S]]
+[[Category: Chatterji D]]
-[[Category: Vijayabaskar, M S]]
+[[Category: Roy S]]
-[[Category: Vijayan, M]]
+[[Category: Vijayabaskar MS]]
-[[Category: Vishveshwara, S]]
+[[Category: Vijayan M]]
-[[Category: Williams, S M]]
+[[Category: Vishveshwara S]]
-[[Category: Dna binding protein]]
+[[Category: Williams SM]]
-[[Category: Ferritin-like fold]]
-[[Category: Ferroxidation]]
-[[Category: Iron]]

4m33

From Proteopedia

Revision as of 10:49, 21 December 2022

Crystal structure of gated-pore mutant H141D of second DNA-Binding protein under starvation from Mycobacterium smegmatis

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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