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1fgb

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TOXIN

Structural highlights

1fgb is a 5 chain structure with sequence from Vibrio cholerae 569B. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHTB_VIBCH The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Publication Abstract from PubMed

Cholera toxin, a heterohexameric AB5 enterotoxin released by Vibrio cholera, induces a profuse secretory diarrhea in susceptible hosts. Choleragenoid, the B subunit pentamer of cholera toxin, directs the enzymatic A subunit to its target by binding the GM1 gangliosides exposed on the luminal surface of intestinal epithelial cells. The crystal structure of choleragenoid has been independently solved and refined at 2.4 A resolution by combining single isomorphous replacement with non-crystallographic symmetry averaging. The structure of the B subunits, and their pentameric arrangement, closely resembles that reported for the intact holotoxin, choleragen, the heat-labile enterotoxin from Escherichia coli, and for a choleragenoid-GM1 pentasaccharide complex. In the absence of the A subunit the central cavity of the B pentamer is a highly solvated channel. The binding of choleragenoid to the A subunit or to its receptor pentasaccharide modestly affects the local stereochemistry without perceptibly altering the subunit interface.

The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid.,Zhang RG, Westbrook ML, Westbrook EM, Scott DL, Otwinowski Z, Maulik PR, Reed RA, Shipley GG J Mol Biol. 1995 Aug 25;251(4):550-62. PMID:7658472^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang RG, Westbrook ML, Westbrook EM, Scott DL, Otwinowski Z, Maulik PR, Reed RA, Shipley GG. The 2.4 A crystal structure of cholera toxin B subunit pentamer: choleragenoid. J Mol Biol. 1995 Aug 25;251(4):550-62. PMID:7658472 doi:http://dx.doi.org/S0022-2836(85)70455-X

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fgb"

Categories: Large Structures | Vibrio cholerae 569B | Westbrook E | Zhang R-G

@@ Line 1: / Line 1: @@
 ==TOXIN==
-<StructureSection load='1fgb' size='340' side='right' caption='[[1fgb]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+<StructureSection load='1fgb' size='340' side='right'caption='[[1fgb]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1fgb]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FGB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1fgb]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_569B Vibrio cholerae 569B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FGB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FGB FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fgb OCA], [http://pdbe.org/1fgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fgb RCSB], [http://www.ebi.ac.uk/pdbsum/1fgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fgb ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fgb OCA], [https://pdbe.org/1fgb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fgb RCSB], [https://www.ebi.ac.uk/pdbsum/1fgb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fgb ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH]] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
+[https://www.uniprot.org/uniprot/CHTB_VIBCH CHTB_VIBCH] The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 ==See Also==
-*[[Cholera toxin|Cholera toxin]]
+*[[Cholera toxin 3D structures|Cholera toxin 3D structures]]
 *[[User:David Solfiell/sandbox 1|User:David Solfiell/sandbox 1]]
 == References ==
@@ Line 25: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Vibrio cholerae]]
+[[Category: Large Structures]]
-[[Category: Westbrook, E]]
+[[Category: Vibrio cholerae 569B]]
-[[Category: Zhang, R G]]
+[[Category: Westbrook E]]
-[[Category: Adp-ribosylation]]
+[[Category: Zhang R-G]]
-[[Category: Cholera toxin]]
-[[Category: Choleragenoid]]
-[[Category: Enterotoxin]]

1fgb

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Current revision

TOXIN

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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