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| | ==Crystal structure of the SpyTag/SpyCatcher complex== | | ==Crystal structure of the SpyTag/SpyCatcher complex== |
| - | <StructureSection load='4mli' size='340' side='right' caption='[[4mli]], [[Resolution|resolution]] 2.10Å' scene=''> | + | <StructureSection load='4mli' size='340' side='right'caption='[[4mli]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mli]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MLI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mli]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MLI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mls|4mls]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mli OCA], [https://pdbe.org/4mli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mli RCSB], [https://www.ebi.ac.uk/pdbsum/4mli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mli ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CnaB2, fba2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mli OCA], [http://pdbe.org/4mli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mli RCSB], [http://www.ebi.ac.uk/pdbsum/4mli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mli ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8G9G1_STRPY Q8G9G1_STRPY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Micrococcus scarlatinae klein 1884]] | + | [[Category: Large Structures]] |
| - | [[Category: Fierer, J O]] | + | [[Category: Streptococcus pyogenes]] |
| - | [[Category: Howarth, M]] | + | [[Category: Fierer JO]] |
| - | [[Category: Li, L]] | + | [[Category: Howarth M]] |
| - | [[Category: Rapoport, T A]] | + | [[Category: Li L]] |
| - | [[Category: Isopeptide bond]] | + | [[Category: Rapoport TA]] |
| - | [[Category: Peptide binding protein]]
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| - | [[Category: Protein engineering]]
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| - | [[Category: Spycatcher]]
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| Structural highlights
Function
Q8G9G1_STRPY
Publication Abstract from PubMed
Peptide tagging is a key strategy for observing and isolating proteins. However, the interactions of proteins with peptides are nearly all rapidly reversible. Proteins tagged with the peptide SpyTag form an irreversible covalent bond to the SpyCatcher protein via a spontaneous isopeptide linkage, thereby offering a genetically encoded way to create peptide interactions that resist force and harsh conditions. Here, we determined the crystal structure of the reconstituted covalent complex of SpyTag and SpyCatcher at 2.1A resolution. The structure showed the expected reformation of the beta-sandwich domain seen in the parental streptococcal adhesin, but flanking sequences at both N- and C-termini of SpyCatcher were disordered. In addition, only 10 out of 13 amino acids of the SpyTag peptide were observed to interact with SpyCatcher, pointing to specific contacts important for rapid split protein reconstitution. Based on these structural insights, we expressed a range of SpyCatcher variants and identified a minimized SpyCatcher, 32 residues shorter, that maintained rapid reaction with SpyTag. Together, these results give insight into split protein beta-strand complementation and enhance a distinct approach to ultrastable molecular interaction.
Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag.,Li L, Fierer JO, Rapoport TA, Howarth M J Mol Biol. 2013 Oct 23. pii: S0022-2836(13)00666-9. doi:, 10.1016/j.jmb.2013.10.021. PMID:24161952[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li L, Fierer JO, Rapoport TA, Howarth M. Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag. J Mol Biol. 2013 Oct 23. pii: S0022-2836(13)00666-9. doi:, 10.1016/j.jmb.2013.10.021. PMID:24161952 doi:http://dx.doi.org/10.1016/j.jmb.2013.10.021
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