Phosphoenolpyruvate carboxykinase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:39, 10 January 2023) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2olr.png|left|200px|thumb|Crystal structure of ATP-driven E. coli PEPCK complex with ATP (stick model), CO2 and Mg+2 ion (green) [[2olr]]]]
+
<StructureSection load='' size='350' side='right' caption='GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene='54/540171/Cv/1'>
-
{{STRUCTURE_2olr| PDB=2olr | SIZE=350| SCENE= |right|CAPTION=ATP-driven E. coli PEPCK complex with ATP, CO2 and Mg+2 [[2olr]] }}
+
 +
== Function ==
 +
'''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO<sub>2</sub>. PEPCK is part of the [[gluconeogenesis]] pathway<ref>PMID:9242918</ref>. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.
 +
See also: [[Gluconeogenesis]].
-
 
+
== Structural highlights ==
-
 
+
''E. coli'' GTP-driven PEPCK <scene name='54/540171/Cv/9'>active site</scene> is located in a pocket at the enzyme surface<ref>PMID:11851336</ref>. Water molecules are shown as red spheres.
-
 
+
*<scene name='54/540171/Cv/10'>Mn coordination site</scene>.
-
 
+
*<scene name='54/540171/Cv/11'>Na coordination site</scene>.
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
'''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO2. PEPCK is part of the gluconeogenesis pathway. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.
+
-
 
+
== 3D Structures of PEPCK ==
== 3D Structures of PEPCK ==
 +
[[Phosphoenolpyruvate carboxykinase 3D structures]]
-
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+
</StructureSection>
-
 
+
-
===PEPCK ATP driven===
+
-
 
+
-
[[1oen]], [[1fiy]] – EcPEPCK - ''Escherichia coli''<br />
+
-
[[1ayl]] – EcPEPCK + ATP + oxalate + Mg<br />
+
-
[[1aq2]] – EcPEPCK + ATP + pyruvate + Mg + Mn<br />
+
-
[[2py7]] – EcPEPCK (mutant) + ATP + Mg + Mn<br />
+
-
[[2olq]] – EcPEPCK + ATP + CO2 + Mg + Mn<br />
+
-
[[2pxz]] – EcPEPCK + ATP + CO2 + oxaloacetate + Mg + Mn<br />
+
-
[[2olr]] – EcPEPCK + ATP + CO2 + Mg <br />
+
-
[[1os1]] – EcPEPCK + ATP + pyruvate + Mg + Ca<br />
+
-
[[1k3c]] – EcPEPCK + ADP + pyruvate + Mg + AlF3<br />
+
-
[[1k3d]] – EcPEPCK + ADP + Mg + AlF3<br />
+
-
[[1j3b]] – TtPEPCK – ''Thermus thermophilus'' <br />
+
-
[[1yvy]] – AnsPEPCK – ''Anaerobiospirillum succiniciproducens''<br />
+
-
[[1ytm]] – AnsPEPCK + ATP + oxalate + Mg + Mn<br />
+
-
[[1xkv]], [[2pc9]] – TtPEPCK + ATP <br />
+
-
 
+
-
===PEPCK GTP driven===
+
-
 
+
-
[[1khb]] – EcPEPCK + GTP analog + Mg <br />
+
-
[[1khe]] – EcPEPCK + GTP analog + Mn <br />
+
-
[[1khf]] – EcPEPCK + PEP + Mn <br />
+
-
[[1khg]] – EcPEPCK + Mn <br />
+
-
[[1m51]], [[1nhx]] – EcPEPCK + inhibitor + Mn<br />
+
-
[[2faf]] – cPEPCK + Mn - chicken<br />
+
-
[[2fah]] – cPEPCK + GDP + Mn <br />
+
-
[[2qzy]] – cPEPCK + PEP + Mn <br />
+
-
[[2gmv]] – hPEPCK + inhibitor + PEP + Mn - human<br />
+
-
[[2qew]] – rPEPCK + Mn - rat<br />
+
-
[[2qey]] – rPEPCK + GTP + Mn<br />
+
-
[[2qf1]] – rPEPCK + oxaloacetate + Mn<br />
+
-
[[2rk7]] – rPEPCK + oxalate + Mn<br />
+
-
[[3dt2]], [[3dt4]] – rPEPCK + GTP + oxalate + Mn<br />
+
-
[[3mof]] – rPEPCK (mutant) + GTP + oxalate + Mn<br />
+
-
[[3dt7]] – rPEPCK + GTP + sulfopyruvate + Mn<br />
+
-
[[3moe]] – rPEPCK (mutant) + GTP + sulfopyruvate + Mn<br />
+
-
[[3dtb]] – rPEPCK + GTP + phosphoglycolate + Mn<br />
+
-
[[3moh]] – rPEPCK (mutant) + GDP + phosphoglycolate + Mn<br />
+
-
[[2rk8]], [[2rka]], [[2rkd]], [[2rke]] – rPEPCK + inhibitor + Mn<br />
+
-
[[2qf2]] – rPEPCK + GDP + oxaloacetate + Mn<br />
+
-
[[2zci]] – PEPCK – ''Corynebacterium glutamicum'' <br />
+
-
 
+
-
[[1ygg]] – AsPEPCK – ''Actinobacillus succinogenes''<br />
+
-
[[1ylh]] – AsPEPCK + pyruvate + Mn<br />
+
 +
== References ==
 +
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry 1khf)

Drag the structure with the mouse to rotate

References

  1. ↑ Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581
  2. ↑ Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky

Retrieved from "http://52.214.119.220/wiki/index.php/Phosphoenolpyruvate_carboxykinase"
Personal tools