Phosphoenolpyruvate carboxykinase

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{{STRUCTURE_2olr| PDB=2olr | SIZE=350| SCENE= |right|CAPTION=ATP-driven E. coli PEPCK complex with ATP (stick model), CO2 and Mg+2 ion (green) [[2olr]] }}
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<StructureSection load='' size='350' side='right' caption='GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene='54/540171/Cv/1'>
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'''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO2. PEPCK is part of the gluconeogenesis pathway. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.
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== Function ==
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'''Phosphoenolpyruvate carboxykinase''' (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO<sub>2</sub>. PEPCK is part of the [[gluconeogenesis]] pathway<ref>PMID:9242918</ref>. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.
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See also: [[Gluconeogenesis]].
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== Structural highlights ==
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''E. coli'' GTP-driven PEPCK <scene name='54/540171/Cv/9'>active site</scene> is located in a pocket at the enzyme surface<ref>PMID:11851336</ref>. Water molecules are shown as red spheres.
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*<scene name='54/540171/Cv/10'>Mn coordination site</scene>.
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*<scene name='54/540171/Cv/11'>Na coordination site</scene>.
== 3D Structures of PEPCK ==
== 3D Structures of PEPCK ==
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[[Phosphoenolpyruvate carboxykinase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Phosphoenolpyruvate carboxykinase ATP driven
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**[[1oen]], [[1fiy]] – EcPEPCK - ''Escherichia coli''<br />
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**[[1ayl]] – EcPEPCK + ATP + oxalate + Mg<br />
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**[[1aq2]] – EcPEPCK + ATP + pyruvate + Mg + Mn<br />
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**[[2py7]] – EcPEPCK (mutant) + ATP + Mg + Mn<br />
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**[[2olq]] – EcPEPCK + ATP + CO2 + Mg + Mn<br />
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**[[2pxz]] – EcPEPCK + ATP + CO2 + oxaloacetate + Mg + Mn<br />
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**[[2olr]] – EcPEPCK + ATP + CO2 + Mg <br />
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**[[1os1]] – EcPEPCK + ATP + pyruvate + Mg + Ca<br />
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**[[1k3c]] – EcPEPCK + ADP + pyruvate + Mg + AlF3<br />
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**[[1k3d]] – EcPEPCK + ADP + Mg + AlF3<br />
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**[[1j3b]] – TtPEPCK – ''Thermus thermophilus'' <br />
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**[[1yvy]] – AnsPEPCK – ''Anaerobiospirillum succiniciproducens''<br />
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**[[1ytm]] – AnsPEPCK + ATP + oxalate + Mg + Mn<br />
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**[[1xkv]], [[2pc9]] – TtPEPCK + ATP <br />
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*Phosphoenolpyruvate carboxykinase GTP driven
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**[[1khf]] – EcPEPCK + PEP + Mn <br />
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**[[1khg]] – EcPEPCK + Mn <br />
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**[[1m51]], [[1nhx]] – EcPEPCK + inhibitor + Mn<br />
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**[[2faf]] – cPEPCK + Mn - chicken<br />
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**[[2qzy]] – cPEPCK + PEP + Mn <br />
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**[[2gmv]] – hPEPCK + inhibitor + PEP + Mn - human<br />
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**[[2qew]] – rPEPCK + Mn - rat<br />
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**[[2qf1]] – rPEPCK + oxaloacetate + Mn<br />
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**[[2rk7]] – rPEPCK + oxalate + Mn<br />
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**[[2rk8]], [[2rka]], [[2rkd]], [[2rke]] – rPEPCK + inhibitor + Mn<br />
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**[[4wiu]] – MtPEPCK + oxalate + Mn – ''Mycobacterium tuberculosis''<br />
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**[[4wie]] – MtPEPCK + Mn <br />
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*PEPCK GTP driven complex with nucleotide
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**[[1khb]] – EcPEPCK + GTP analog + Mg <br />
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**[[1khe]] – EcPEPCK + GTP analog + Mn <br />
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**[[2fah]] – cPEPCK + GDP + Mn <br />
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**[[2qey]] – rPEPCK + GTP + Mn<br />
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**[[4gnm]], [[4gnq]] - rPEPCK + oxalate + GTP + Mn<br />
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**[[4gmw]], [[4gnl]], [[4gnp]] – rPEPCK + PEP + GDP + Mn <br />
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**[[3dt2]], [[3dt4]], [[4gmu]] – rPEPCK + GTP + oxalate + Mn<br />
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**[[3mof]] – rPEPCK (mutant) + GTP + oxalate + Mn<br />
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**[[3dt7]], [[4gmm]], [[4gmz]], [[4gno]] – rPEPCK + GTP + sulfopyruvate + Mn<br />
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**[[3moe]], [[4ox2]] – rPEPCK (mutant) + GTP + sulfopyruvate + Mn<br />
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**[[3dtb]] – rPEPCK + GTP + phosphoglycolate + Mn<br />
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**[[3moh]] – rPEPCK (mutant) + GDP + phosphoglycolate + Mn<br />
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**[[2qf2]] – rPEPCK + GDP + oxaloacetate + Mn<br />
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**[[4rcg]] – MtPEPCK + GDP + Mg <br />
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**[[4r43]] – MtPEPCK + GDP + Mn <br />
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*PEPCK
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**[[2zci]] – PEPCK – ''Corynebacterium glutamicum'' <br />
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== References ==
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**[[1ygg]] – AsPEPCK – ''Actinobacillus succinogenes''<br />
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<references/>
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**[[1ylh]] – AsPEPCK + pyruvate + Mn<br />
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**[[1ii2]] – PEPCK – ''Trypanosoma cruzi''<br />
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}}
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[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry 1khf)

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References

  1. ↑ Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581
  2. ↑ Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

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