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1iaw

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Revision as of 08:05, 11 January 2023

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

Structural highlights

1iaw is a 6 chain structure with sequence from Lentzea aerocolonigenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2N1_LENAE An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.^[1] ^[2] ^[3]

Publication Abstract from PubMed

NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.

Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement.,Huai Q, Colandene JD, Topal MD, Ke H Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jo K, Topal MD. DNA topoisomerase and recombinase activities in Nae I restriction endonuclease. Science. 1995 Mar 24;267(5205):1817-20. doi: 10.1126/science.7892605. PMID:7892605 doi:http://dx.doi.org/10.1126/science.7892605
↑ Jo K, Topal MD. Effects on NaeI-DNA recognition of the leucine to lysine substitution that transforms restriction endonuclease NaeI to a topoisomerase: a model for restriction endonuclease evolution. Nucleic Acids Res. 1996 Nov 1;24(21):4171-5. doi: 10.1093/nar/24.21.4171. PMID:8932368 doi:http://dx.doi.org/10.1093/nar/24.21.4171
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Kruger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 1;31(7):1805-12. PMID:12654995
↑ Huai Q, Colandene JD, Topal MD, Ke H. Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement. Nat Struct Biol. 2001 Aug;8(8):665-9. PMID:11473254 doi:10.1038/90366

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1iaw"

@@ Line 3: / Line 3: @@
 <StructureSection load='1iaw' size='340' side='right'caption='[[1iaw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1iaw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IAW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1iaw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IAW FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ev7|1ev7]]</div></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iaw OCA], [https://pdbe.org/1iaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iaw RCSB], [https://www.ebi.ac.uk/pdbsum/1iaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iaw ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iaw OCA], [https://pdbe.org/1iaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iaw RCSB], [https://www.ebi.ac.uk/pdbsum/1iaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iaw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/T2N1_NOCAE T2N1_NOCAE]] Recognizes the double-stranded unmethylated sequence GCCGGC and cleaves after C-3.
+[https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 25: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Type II site-specific deoxyribonuclease]]
+[[Category: Lentzea aerocolonigenes]]
-[[Category: Colandene, J D]]
+[[Category: Colandene JD]]
-[[Category: Huai, Q]]
+[[Category: Huai Q]]
-[[Category: Ke, H]]
+[[Category: Ke H]]
-[[Category: Topal, M D]]
+[[Category: Topal MD]]
-[[Category: Hydrolase-dna complex]]
-[[Category: Protein-dna complex]]

1iaw

From Proteopedia

Revision as of 08:05, 11 January 2023

CRYSTAL STRUCTURE OF NAEI COMPLEXED WITH 17MER DNA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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