Malate synthase

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(New page: <StructureSection load='1n8w' size='350' side='right' caption='Structure of malate synthase complex with CoA, glyoxylate and Mg+2 ion (PDB entry 1n8w)' scene=''> '''Malate syntha...)
Current revision (09:03, 11 January 2023) (edit) (undo)
 
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<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
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== Function ==
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'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomenclature|(S)]]-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
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<StructureSection load='1n8w' size='350' side='right' caption='Structure of malate synthase complex with CoA, glyoxylate and Mg+2 ion (PDB entry [[1n8w]])' scene=''>
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See also: [[Glyoxylate cycle]]
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'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. '''MS G''' is formed during growth on glycolate and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
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==3d structures of malate synthase==
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== Structural highlights ==
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<scene name='57/573146/Cv/8'>MS active site pocket is situated between the TIM barrel and the C-terminal</scene>. The ternary complex contains malate, acetyl-CoA and Mg+2 ion<ref>PMID:16877713</ref>.
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===Malate synthase G===
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<scene name='57/573146/Cv/9'>Malate/Mg+2 ion binding site</scene>. Water molecules are shown as red spheres.
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[[1y8b]], [[2jqx]] – EcMS (mutant) – ''Escherichia coli'' - NMR<br />
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<scene name='57/573146/Cv/10'>Acetyl-CoA binding site</scene>.
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[[4ex4]] – MS + Mg – ''Mycobacterium leprae''<br />
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===Malate synthase G binary complex===
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==3d structures of malate synthase==
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[[Malate synthase 3D structures]]
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[[1d8c]] – EcMS (mutant) + glyoxylate + Mg <br />
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[[1n8i]] – MtMS + glyoxylate + Mg – ''Mycobacterium tuberculosis''<br />
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[[3s9i]], [[3s9z]], [[3sad]], [[3saz]], [[3sb0]] – MtMS (mutant) + inhibitor + Mg <br />
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===Malate synthase G ternary complex===
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</StructureSection>
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[[1p7t]] – EcMS (mutant) + pyruvate + Mg + acetyl-CoA<br />
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== References ==
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[[1n8w]] – MtMS + glyoxylate + Mg + CoA<br />
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<references/>
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[[2gq3]] – MtMS + malate + Mg + CoA<br />
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[[Category:Topic Page]]

Current revision

Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry 2gq3)

Drag the structure with the mouse to rotate

References

  1. ↑ Anstrom DM, Kallio K, Remington SJ. Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution. Protein Sci. 2003 Sep;12(9):1822-32. PMID:12930982
  2. ↑ Anstrom DM, Remington SJ. The product complex of M. tuberculosis malate synthase revisited. Protein Sci. 2006 Aug;15(8):2002-7. PMID:16877713 doi:15/8/2002

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Karsten Theis, Joel L. Sussman

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