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Malate synthase

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<StructureSection load='2gq3' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
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<StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'>
== Function ==
== Function ==
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'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
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'''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomenclature|(S)]]-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate.
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See also: [[Glyoxylate cycle]]
== Structural highlights ==
== Structural highlights ==
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<scene name='57/573146/Cv/7'>MS active site pocket is situated between the TIM barrel and the C-terminal</scene>. The ternary complex contains malate, acetyl-CoA and Mg+2 ion<ref>PMID:16877713</ref>.
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<scene name='57/573146/Cv/8'>MS active site pocket is situated between the TIM barrel and the C-terminal</scene>. The ternary complex contains malate, acetyl-CoA and Mg+2 ion<ref>PMID:16877713</ref>.
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<scene name='57/573146/Cv/3'>Malate/Mg+2 ion binding site</scene>. Water molecules shown as red spheres.
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<scene name='57/573146/Cv/9'>Malate/Mg+2 ion binding site</scene>. Water molecules are shown as red spheres.
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<scene name='57/573146/Cv/6'>Acetyl-CoA binding site</scene>.
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<scene name='57/573146/Cv/10'>Acetyl-CoA binding site</scene>.
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</StructureSection>
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==3d structures of malate synthase==
==3d structures of malate synthase==
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[[Malate synthase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*'''Malate synthase'''
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**[[1y8b]], [[2jqx]] – EcMS G (mutant) – ''Escherichia coli'' - NMR<br />
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**[[3cuz]], [[3cv1]] – EcMS A<br />
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**[[4ex4]] – MS G + Mg – ''Mycobacterium leprae''<br />
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**[[5h8p]] – MtMS G – ''Mycobacterium tuberculosis''<br />
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**[[5h8m]] – MtMS G (mutant) + Mg <br />
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**[[3cux]] – MS + Mg – ''Bacillus anthracis''<br />
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**[[5oas]] – PaMS G + Mg – ''Pseudomonas aeruginosa''<br />
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*Malate synthase binary complex
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**[[1d8c]] – EcMS G (mutant) + glyoxylate + Mg <br />
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**[[3cv2]] – EcMS A + oxalate + Mg <br />
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**[[1n8i]] – MtMS G + glyoxylate + Mg <br />
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**[[5h8u]] – MtMS G + malate + Mg <br />
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**[[56axe]] – MtMS G + acetyl CoA + Mg <br />
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**[[5c7v]], [[5cah]], [[5cjm]], [[5e9x]] – MtMS G (mutant) + pyrrole derivative + Mg <br />
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**[[5cew]] – MtMS G (mutant) + pyridine derivative + Mg <br />
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**[[5cjn]] – MtMS G (mutant) + quinoxaline derivative + Mg <br />
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**[[5dx7]] – MtMS G (mutant) + isoxazole derivative + Mg <br />
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**[[5c9r]], [[5c9u]], [[5c9w]], [[5cbj]], [[5ccz]] – MtMS G (mutant) + phenyl derivative + Mg <br />
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**[[5c9x]], [[5cbi]] – MtMS G (mutant) + benzoate derivative + Mg <br />
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**[[5cak]], [[5cbb]], [[5cc3]], [[5cc5]], [[5cc6]], [[5cc7]], [[5drc]], [[5dri]], [[5ecv]] – MtMS G (mutant) + indole derivative + Mg <br />
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**[[5t8g]] – MtMS G (mutant) + thiophene derivative + Mg <br />
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**[[3s9i]], [[3s9z]], [[3sad]], [[3saz]], [[3sb0]] – MtMS G (mutant) + inhibitor + Mg <br />
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**[[3oyx]], [[3pug]] – HvMS G + glyoxylate + Mg – ''Haloferax volcanii''<br />
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**[[5tao]] – HvMS G + glyoxylate + Pb<br />
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**[[5vfb]] – PaMS + glycolate + Ni<br />
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*Malate synthase ternary complex
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**[[1p7t]] – EcMS G (mutant) + pyruvate + Mg + acetyl-CoA<br />
 
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**[[3oyz]] – HvMS + pyruvate + Mg + acetyl-CoA<br />
 
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**[[1n8w]] – MtMS G + glyoxylate + Mg + CoA<br />
 
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**[[2gq3]] – MtMS G + malate + Mg + CoA<br />
 
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry 2gq3)

Drag the structure with the mouse to rotate

References

  1. ↑ Anstrom DM, Kallio K, Remington SJ. Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution. Protein Sci. 2003 Sep;12(9):1822-32. PMID:12930982
  2. ↑ Anstrom DM, Remington SJ. The product complex of M. tuberculosis malate synthase revisited. Protein Sci. 2006 Aug;15(8):2002-7. PMID:16877713 doi:15/8/2002

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Michal Harel, Alexander Berchansky, Karsten Theis, Joel L. Sussman

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