8d4b

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'''Unreleased structure'''
 
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The entry 8d4b is ON HOLD until Paper Publication
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==Structure of Cas12a2 ternary complex==
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<StructureSection load='8d4b' size='340' side='right'caption='[[8d4b]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8d4b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfuricurvum_sp._PC08-66 Sulfuricurvum sp. PC08-66] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D4B FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d4b OCA], [https://pdbe.org/8d4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d4b RCSB], [https://www.ebi.ac.uk/pdbsum/8d4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d4b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0C2W1L1_9PROT A0A0C2W1L1_9PROT]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded DNA following recognition of a complementary RNA target, culminating in abortive infection(1). Here we report structures of Cas12a2 in binary, ternary and quaternary complexes to reveal a complete activation pathway. Our structures reveal that Cas12a2 is autoinhibited until binding a cognate RNA target, which exposes the RuvC active site within a large, positively charged cleft. Double-stranded DNA substrates are captured through duplex distortion and local melting, stabilized by pairs of 'aromatic clamp' residues that are crucial for double-stranded DNA degradation and in vivo immune system function. Our work provides a structural basis for this mechanism of abortive infection to achieve population-level immunity, which can be leveraged to create rational mutants that degrade a spectrum of collateral substrates.
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Authors: Bravo, J.P.K., Taylor, D.W.
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RNA targeting unleashes indiscriminate nuclease activity of CRISPR-Cas12a2.,Bravo JPK, Hallmark T, Naegle B, Beisel CL, Jackson RN, Taylor DW Nature. 2023 Jan 4. doi: 10.1038/s41586-022-05560-w. PMID:36599980<ref>PMID:36599980</ref>
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Description: Structure of Cas12a2 ternary complex
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Bravo, J.P.K]]
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<div class="pdbe-citations 8d4b" style="background-color:#fffaf0;"></div>
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[[Category: Taylor, D.W]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sulfuricurvum sp. PC08-66]]
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[[Category: Synthetic construct]]
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[[Category: Bravo JPK]]
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[[Category: Taylor DW]]

Revision as of 07:30, 18 January 2023

Structure of Cas12a2 ternary complex

PDB ID 8d4b

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