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8hbr
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The C-terminal domain of Spiral2== |
| + | <StructureSection load='8hbr' size='340' side='right'caption='[[8hbr]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8hbr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Physcomitrium_patens Physcomitrium patens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HBR FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hbr OCA], [https://pdbe.org/8hbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hbr RCSB], [https://www.ebi.ac.uk/pdbsum/8hbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hbr ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A2K1IUB4_PHYPA A0A2K1IUB4_PHYPA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plant cells form microtubule arrays, called ;cortical microtubules', beneath the plasma membrane which are critical for cell-wall organization and directional cell growth. Cortical microtubules are nucleated independently of centrosomes. Spiral2 is a land-plant-specific microtubule minus-end-targeting protein that stabilizes the minus ends by inhibiting depolymerization of the filament. Spiral2 possesses an N-terminal microtubule-binding domain and a conserved C-terminal domain whose function is unknown. In this study, the crystal structure of the conserved C-terminal domain of Spiral2 was determined using the single-wavelength anomalous dispersion method. Refinement of the model to a resolution of 2.2 A revealed a helix-turn-helix fold with seven alpha-helices. The protein crystallized as a dimer, but SEC-MALS analysis showed the protein to be monomeric. A structural homology search revealed that the protein has similarity to the C-terminal domain of the katanin regulatory subunit p80. The structure presented here suggests that the C-terminal domain of Spiral2 represents a new class of microtubule dynamics modulator across the kingdom. | ||
| - | + | Crystal structure of the C-terminal domain of the plant-specific microtubule-associated protein Spiral2.,Ohno M, Higuchi Y, Hayashi I Acta Crystallogr F Struct Biol Commun. 2023 Jan 1;79(Pt 1):17-22. doi: , 10.1107/S2053230X22011815. Epub 2023 Jan 1. PMID:36598352<ref>PMID:36598352</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8hbr" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Physcomitrium patens]] | ||
| + | [[Category: Hayashi I]] | ||
Current revision
The C-terminal domain of Spiral2
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