This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
8cy8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 8cy8 is ON HOLD until Paper Publication Authors: Zheng, S., Guddat, L.W. Description: apo form Cryo-EM structure of Campylobacter jejune ketol-acid...) |
|||
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==apo form Cryo-EM structure of Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde== | |
| - | + | <StructureSection load='8cy8' size='340' side='right'caption='[[8cy8]], [[Resolution|resolution]] 2.94Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[8cy8]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CY8 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTD:PENTANEDIAL'>PTD</scene></td></tr> | |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cy8 OCA], [https://pdbe.org/8cy8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cy8 RCSB], [https://www.ebi.ac.uk/pdbsum/8cy8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cy8 ProSAT]</span></td></tr> |
| - | [[Category: | + | </table> |
| - | [[Category: Guddat | + | == Function == |
| + | [https://www.uniprot.org/uniprot/A0A5T0UG45_CAMJU A0A5T0UG45_CAMJU] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.[ARBA:ARBA00002172][HAMAP-Rule:MF_00435] | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Campylobacter jejuni]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Guddat LW]] | ||
| + | [[Category: Zheng S]] | ||
Current revision
apo form Cryo-EM structure of Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde
| |||||||||||
