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4pa1

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Revision as of 12:49, 1 February 2023

Crystal Structure of Catalytic Core domain of FIV Integrase

Structural highlights

4pa1 is a 1 chain structure with sequence from Feline immunodeficiency virus (isolate Petaluma). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

POL_FIVPE During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Publication Abstract from PubMed

Retroviral DNA integration into the host genome is mediated by nucleoprotein assemblies containing tetramers of viral integrase (IN). Whereas the fully active form of IN comprises a dimer of dimers, the molecular basis of IN multimerization has not been fully characterized. IN has consistently been crystallized in an analogous dimeric form in all crystallographic structures and experimental evidence as to the level of similarity between IN monomeric and dimeric conformations is missing because of the lack of IN monomeric structures. Here we identify Phe187 as a critical dimerization determinant of IN from feline immunodeficiency virus (FIV), a nonprimate lentivirus that causes AIDS in the natural host, and report, in addition to a canonical dimeric structure of the FIV IN core-domain, a monomeric structure revealing the preservation of the backbone structure between the two multimeric forms and suggest a role for Phe187 in "hinging" the flexible IN dimer.

Identification of phe187 as a crucial dimerization determinant facilitates crystallization of a monomeric retroviral integrase core domain.,Galilee M, Alian A Structure. 2014 Oct 7;22(10):1512-9. doi: 10.1016/j.str.2014.08.001. Epub 2014, Sep 4. PMID:25199694^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Galilee M, Alian A. Identification of phe187 as a crucial dimerization determinant facilitates crystallization of a monomeric retroviral integrase core domain. Structure. 2014 Oct 7;22(10):1512-9. doi: 10.1016/j.str.2014.08.001. Epub 2014, Sep 4. PMID:25199694 doi:http://dx.doi.org/10.1016/j.str.2014.08.001

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pa1"

Categories: Large Structures | Alian A | Galilee M

@@ Line 3: / Line 3: @@
 <StructureSection load='4pa1' size='340' side='right'caption='[[4pa1]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4pa1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fiv Fiv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PA1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4pa1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Feline_immunodeficiency_virus_(isolate_Petaluma) Feline immunodeficiency virus (isolate Petaluma)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PA1 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mq3|4mq3]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa1 OCA], [https://pdbe.org/4pa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pa1 RCSB], [https://www.ebi.ac.uk/pdbsum/4pa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa1 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11674 FIV])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa1 OCA], [http://pdbe.org/4pa1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pa1 RCSB], [http://www.ebi.ac.uk/pdbsum/4pa1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa1 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/POL_FIVPE POL_FIVPE]] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
+[https://www.uniprot.org/uniprot/POL_FIVPE POL_FIVPE] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 19: @@
 ==See Also==
-*[[Retroviral Integrase|Retroviral Integrase]]
+*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Fiv]]
 [[Category: Large Structures]]
-[[Category: Alian, A]]
+[[Category: Alian A]]
-[[Category: Galilee, M]]
+[[Category: Galilee M]]
-[[Category: Integrase]]
-[[Category: Retrovirus]]
-[[Category: Viral protein]]

4pa1

From Proteopedia

Revision as of 12:49, 1 February 2023

Crystal Structure of Catalytic Core domain of FIV Integrase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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