|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==TatA T22P== | | ==TatA T22P== |
| - | <StructureSection load='2lzr' size='340' side='right' caption='[[2lzr]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2lzr' size='340' side='right'caption='[[2lzr]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lzr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LZR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LZR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lzr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LZR FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b3836, JW3813, mttA1, tatA, yigT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lzr OCA], [https://pdbe.org/2lzr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lzr RCSB], [https://www.ebi.ac.uk/pdbsum/2lzr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lzr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lzr OCA], [http://pdbe.org/2lzr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lzr RCSB], [http://www.ebi.ac.uk/pdbsum/2lzr PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TATA_ECOLI TATA_ECOLI]] Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.<ref>PMID:9649434</ref> <ref>PMID:11922668</ref> | + | [https://www.uniprot.org/uniprot/TATA_ECOLI TATA_ECOLI] Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.<ref>PMID:9649434</ref> <ref>PMID:11922668</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Berks, B C]] | + | [[Category: Large Structures]] |
| - | [[Category: Rodriguez, F M]] | + | [[Category: Berks BC]] |
| - | [[Category: Schnell, J R]] | + | [[Category: Rodriguez FM]] |
| - | [[Category: Dpc]] | + | [[Category: Schnell JR]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Tata]]
| + | |
| Structural highlights
Function
TATA_ECOLI Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.[1] [2]
Publication Abstract from PubMed
The twin-arginine translocase (Tat) carries out the remarkable process of translocating fully folded proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of plant chloroplasts. Tat is required for bacterial pathogenesis and for photosynthesis in plants. TatA, the protein-translocating element of the Tat system, is a small transmembrane protein that assembles into ring-like oligomers of variable size. We have determined a structural model of the Escherichia coli TatA complex in detergent solution by NMR. TatA assembly is mediated entirely by the transmembrane helix. The amphipathic helix extends outwards from the ring of transmembrane helices, permitting assembly of complexes with variable subunit numbers. Transmembrane residue Gln8 points inward, resulting in a short hydrophobic pore in the center of the complex. Simulations of the TatA complex in lipid bilayers indicate that the short transmembrane domain distorts the membrane. This finding suggests that TatA facilitates protein transport by sensitizing the membrane to transient rupture.
Structural model for the protein-translocating element of the twin-arginine transport system.,Rodriguez F, Rouse SL, Tait CE, Harmer J, De Riso A, Timmel CR, Sansom MS, Berks BC, Schnell JR Proc Natl Acad Sci U S A. 2013 Mar 7. PMID:23471988[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sargent F, Bogsch EG, Stanley NR, Wexler M, Robinson C, Berks BC, Palmer T. Overlapping functions of components of a bacterial Sec-independent protein export pathway. EMBO J. 1998 Jul 1;17(13):3640-50. PMID:9649434 doi:10.1093/emboj/17.13.3640
- ↑ Ize B, Gerard F, Zhang M, Chanal A, Voulhoux R, Palmer T, Filloux A, Wu LF. In vivo dissection of the Tat translocation pathway in Escherichia coli. J Mol Biol. 2002 Mar 29;317(3):327-35. PMID:11922668 doi:10.1006/jmbi.2002.5431
- ↑ Rodriguez F, Rouse SL, Tait CE, Harmer J, De Riso A, Timmel CR, Sansom MS, Berks BC, Schnell JR. Structural model for the protein-translocating element of the twin-arginine transport system. Proc Natl Acad Sci U S A. 2013 Mar 7. PMID:23471988 doi:http://dx.doi.org/10.1073/pnas.1219486110
|
