8d04

Publication Abstract from PubMed

Deep learning generative approaches provide an opportunity to broadly explore protein structure space beyond the sequences and structures of natural proteins. Here we use deep network hallucination to generate a wide range of symmetric protein homo-oligomers given only a specification of the number of protomers and the protomer length. Crystal structures of 7 designs are very close to the computational models (median RMSD: 0.6 A), as are 3 cryoEM structures of giant 10 nanometer rings with up to 1550 residues and C33 symmetry; all differ considerably from previously solved structures. Our results highlight the rich diversity of new protein structures that can be generated using deep learning, and pave the way for the design of increasingly complex components for nanomachines and biomaterials.

Hallucinating symmetric protein assemblies.,Wicky BIM, Milles LF, Courbet A, Ragotte RJ, Dauparas J, Kinfu E, Tipps S, Kibler RD, Baek M, DiMaio F, Li X, Carter L, Kang A, Nguyen H, Bera AK, Baker D Science. 2022 Sep 15:eadd1964. doi: 10.1126/science.add1964. PMID:36108048^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.