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4r9i

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Revision as of 06:46, 2 March 2023

Crystal structure of cysteine proteinase inhibitor Serpin18 from Bombyx mori

Structural highlights

4r9i is a 1 chain structure with sequence from Bombyx mori. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C0J8G7_BOMMO

Publication Abstract from PubMed

Serpins generally serve as inhibitors that utilize a mobile reactive center loop (RCL) as bait to trap protease targets. Here, we present the crystal structure of serpin18 from Bombyx mori at 1.65 A resolution, which has a very short and stable RCL. Activity analysis showed that the inhibitory target of serpin18 is a cysteine protease rather than a serine protease. Notably, this inhibitiory reaction results from the formation of an intermediate complex, which then follows for the digestion of protease and inhibitor into small fragments. This activity differs from previously reported modes of inhibition for serpins. Our findings have thus provided novel structural insights into the unique inhibitory mechanism of serpin18. Furthermore, one physiological target of serpin18, fibroinase, was identified, which enables us to better define the potential role for serpin18 in regulating fibroinase activity during B. mori development.

Structural insights into the unique inhibitory mechanism of the silkworm protease inhibitor serpin18.,Guo PC, Dong Z, Zhao P, Zhang Y, He H, Tan X, Zhang W, Xia Q Sci Rep. 2015 Jul 7;5:11863. doi: 10.1038/srep11863. PMID:26148664^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Guo PC, Dong Z, Zhao P, Zhang Y, He H, Tan X, Zhang W, Xia Q. Structural insights into the unique inhibitory mechanism of the silkworm protease inhibitor serpin18. Sci Rep. 2015 Jul 7;5:11863. doi: 10.1038/srep11863. PMID:26148664 doi:http://dx.doi.org/10.1038/srep11863

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4r9i"

@@ Line 3: / Line 3: @@
 <StructureSection load='4r9i' size='340' side='right'caption='[[4r9i]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4r9i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R9I FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4r9i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R9I FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r9i OCA], [http://pdbe.org/4r9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r9i RCSB], [http://www.ebi.ac.uk/pdbsum/4r9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r9i ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r9i OCA], [https://pdbe.org/4r9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r9i RCSB], [https://www.ebi.ac.uk/pdbsum/4r9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r9i ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/C0J8G7_BOMMO C0J8G7_BOMMO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 18: / Line 20: @@
 ==See Also==
-*[[Serpin|Serpin]]
+*[[Serpin 3D structures|Serpin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bommo]]
+[[Category: Bombyx mori]]
 [[Category: Large Structures]]
-[[Category: Guo, P C]]
+[[Category: Guo PC]]
-[[Category: He, H W]]
+[[Category: He HW]]
-[[Category: Xia, Q Y]]
+[[Category: Xia QY]]
-[[Category: Zhao, P]]
+[[Category: Zhao P]]
-[[Category: Hydrolase inhibitor]]
-[[Category: Proteinase inhibitor]]

4r9i

From Proteopedia

Revision as of 06:46, 2 March 2023

Crystal structure of cysteine proteinase inhibitor Serpin18 from Bombyx mori

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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