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| | <StructureSection load='4rj9' size='340' side='right'caption='[[4rj9]], [[Resolution|resolution]] 1.63Å' scene=''> | | <StructureSection load='4rj9' size='340' side='right'caption='[[4rj9]], [[Resolution|resolution]] 1.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4rj9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RJ9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4rj9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RJ9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Os02g0327000, OsGAP1, OSJNBb0042G06.10, OsJ_06508, P0476C12.36 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rj9 OCA], [https://pdbe.org/4rj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rj9 RCSB], [https://www.ebi.ac.uk/pdbsum/4rj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rj9 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rj9 OCA], [http://pdbe.org/4rj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rj9 RCSB], [http://www.ebi.ac.uk/pdbsum/4rj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rj9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GAP1_ORYSJ GAP1_ORYSJ]] Mediates the transient calcium-dependent interaction of PYR/PYL/RCAR abscisic acid (ABA) receptors with the plasma membrane and thus regulates ABA sensitivity (By similarity). Stimulates the GTPase/ATPase activities of YchF1, and regulates its subcellular localization. Promotes tolerance towards salinity stress by limiting the accumulation of reactive oxygen species (ROS) (PubMed:19086295, PubMed:23550829). Promotes resistance to bacterial pathogens (e.g. Xanthomonas oryzae pv. oryzae and P. syringae pv. tomato DC3000) (PubMed:19086295).[UniProtKB:Q9LVH4]<ref>PMID:19086295</ref> <ref>PMID:23550829</ref> | + | [https://www.uniprot.org/uniprot/GAP1_ORYSJ GAP1_ORYSJ] Mediates the transient calcium-dependent interaction of PYR/PYL/RCAR abscisic acid (ABA) receptors with the plasma membrane and thus regulates ABA sensitivity (By similarity). Stimulates the GTPase/ATPase activities of YchF1, and regulates its subcellular localization. Promotes tolerance towards salinity stress by limiting the accumulation of reactive oxygen species (ROS) (PubMed:19086295, PubMed:23550829). Promotes resistance to bacterial pathogens (e.g. Xanthomonas oryzae pv. oryzae and P. syringae pv. tomato DC3000) (PubMed:19086295).[UniProtKB:Q9LVH4]<ref>PMID:19086295</ref> <ref>PMID:23550829</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Japanese rice]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fong, Y H]] | + | [[Category: Oryza sativa Japonica Group]] |
| - | [[Category: Lam, H M]] | + | [[Category: Fong YH]] |
| - | [[Category: Miao, R]] | + | [[Category: Lam HM]] |
| - | [[Category: Wong, K B]] | + | [[Category: Miao R]] |
| - | [[Category: Beta_sandwich]] | + | [[Category: Wong KB]] |
| - | [[Category: Calcium-dependent]]
| + | |
| - | [[Category: Cytosol]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Lipid selectivity]]
| + | |
| - | [[Category: Membrane targeting]]
| + | |
| - | [[Category: Phospholipid binding]]
| + | |
| Structural highlights
Function
GAP1_ORYSJ Mediates the transient calcium-dependent interaction of PYR/PYL/RCAR abscisic acid (ABA) receptors with the plasma membrane and thus regulates ABA sensitivity (By similarity). Stimulates the GTPase/ATPase activities of YchF1, and regulates its subcellular localization. Promotes tolerance towards salinity stress by limiting the accumulation of reactive oxygen species (ROS) (PubMed:19086295, PubMed:23550829). Promotes resistance to bacterial pathogens (e.g. Xanthomonas oryzae pv. oryzae and P. syringae pv. tomato DC3000) (PubMed:19086295).[UniProtKB:Q9LVH4][1] [2]
Publication Abstract from PubMed
The C2 domain is one of the most diverse phospholipid-binding domains mediating cellular signaling. One group of C2-domain proteins are plant-specific and are characterized by their small sizes and simple structures. We have previously reported that a member of this group, OsGAP1, is able to alleviate salt stress and stimulate defense responses, and bind to both phospholipids and an unconventional G-protein, OsYchF1. Here we solved the crystal structure of OsGAP1 to a resolution of 1.63 A. Using site-directed mutagenesis, we successfully differentiated between the clusters of surface residues that are required for binding to phospholipids versus OsYchF1, which, in turn, is critical for its role in stimulating defense responses. On the other hand, the ability to alleviate salt stress by OsGAP1 is dependent only on its ability to bind OsYchF1 and is independent of its phospholipid-binding activity.
Site-Directed Mutagenesis Shows the Significance of Interactions with Phospholipids and the G-protein OsYchF1 on the Physiological Functions of the Rice GTPase-Activating Protein 1 (OsGAP1).,Yung YL, Cheung MY, Miao R, Fong YH, Li KP, Yu MH, Chye ML, Wong KB, Lam HM J Biol Chem. 2015 Aug 18. pii: jbc.M115.655639. PMID:26286751[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cheung MY, Zeng NY, Tong SW, Li WY, Xue Y, Zhao KJ, Wang C, Zhang Q, Fu Y, Sun Z, Sun SS, Lam HM. Constitutive expression of a rice GTPase-activating protein induces defense responses. New Phytol. 2008 Jul;179(2):530-45. PMID:19086295
- ↑ Cheung MY, Li MW, Yung YL, Wen CQ, Lam HM. The unconventional P-loop NTPase OsYchF1 and its regulator OsGAP1 play opposite roles in salinity stress tolerance. Plant Cell Environ. 2013 Nov;36(11):2008-20. doi: 10.1111/pce.12108. Epub 2013, Apr 25. PMID:23550829 doi:http://dx.doi.org/10.1111/pce.12108
- ↑ Yung YL, Cheung MY, Miao R, Fong YH, Li KP, Yu MH, Chye ML, Wong KB, Lam HM. Site-Directed Mutagenesis Shows the Significance of Interactions with Phospholipids and the G-protein OsYchF1 on the Physiological Functions of the Rice GTPase-Activating Protein 1 (OsGAP1). J Biol Chem. 2015 Aug 18. pii: jbc.M115.655639. PMID:26286751 doi:http://dx.doi.org/10.1074/jbc.M115.655639
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