Galactokinase
From Proteopedia
(Difference between revisions)
| (9 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | <StructureSection load='1wuu' size=' | + | <StructureSection load='1wuu' size='400' side='right' caption='Se-Met human GALK complex with galactopyranose, AMPPNP and Mg+2 ion (green) (PDB code [[1wuu]].' scene='86/864099/Cv/1'> |
__TOC__ | __TOC__ | ||
== Function == | == Function == | ||
| Line 11: | Line 11: | ||
== Structural highlights == | == Structural highlights == | ||
| - | The 3D structure of human GALK shows the sugar binding active site wedged between the N- and C-termini of the enzyme. The galactose forms numerous interactions with GALK termini residues and in particular forms hydrogen bonds with an Asp and Arg residues which are conserved in eukaryotic and prokaryotic GALK<ref>PMID:15590630</ref>. | + | The 3D structure of human GALK shows the sugar binding active site wedged between the N- and C-termini of the enzyme. The galactose forms <scene name='86/864099/Cv/5'>numerous interactions</scene> with GALK termini residues and in <scene name='86/864099/Cv/6'>particular forms hydrogen bonds with an Asp and Arg residues which are conserved in eukaryotic and prokaryotic GALK</scene><ref>PMID:15590630</ref>. |
| + | *<scene name='86/864099/Cv/7'>Mg coordination site</scene>. | ||
| + | *<scene name='86/864099/Cv/9'>AMPPNP binding site</scene>. | ||
| + | *<scene name='86/864099/Cv/10'>Whole binding site</scene>. | ||
==3D structures of galactokinase== | ==3D structures of galactokinase== | ||
| Line 18: | Line 21: | ||
[[6gr2]] - hGALK + ADP + gal - human<br /> | [[6gr2]] - hGALK + ADP + gal - human<br /> | ||
| + | [[7rcl]], [[7rcm]] - hGALK (mutant) + ADP + gal <br /> | ||
[[1wuu]] - hGALK + Mg + AMPPNP + gal<br /> | [[1wuu]] - hGALK + Mg + AMPPNP + gal<br /> | ||
[[6q3w]], [[6q8z]] - hGALK + pyrazine derivative + gal<br /> | [[6q3w]], [[6q8z]] - hGALK + pyrazine derivative + gal<br /> | ||
[[6q91]] - hGALK + benzamide derivative + gal<br /> | [[6q91]] - hGALK + benzamide derivative + gal<br /> | ||
[[6q90]], [[6zgw]] - hGALK + pyridine derivative + gal<br /> | [[6q90]], [[6zgw]] - hGALK + pyridine derivative + gal<br /> | ||
| - | [[6q3x]] - hGALK + quinazoline derivative + gal<br /> | + | [[6q3x]], [[6zfh]], [[7s49]] - hGALK + quinazoline derivative + gal<br /> |
| - | [[6zgv]], [[6zgx]], [[6zgz]], [[6zgy]] - hGALK + pyrimidine derivative + gal<br /> | + | [[6zgv]], [[6zgx]], [[6zgz]], [[6zgy]], [[6zh0]] - hGALK + pyrimidine derivative + gal<br /> |
| + | [[7s4c]] - hGALK (mutant) + pyrimidine derivative + gal<br /> | ||
[[6qje]] - hGALK + imidazole derivative + gal<br /> | [[6qje]] - hGALK + imidazole derivative + gal<br /> | ||
| + | [[7ozx]] - hGALK + inhibitor + gal<br /> | ||
[[2aj4]] - GALK + Mg + AMPPNP + gal - yeast<br /> | [[2aj4]] - GALK + Mg + AMPPNP + gal - yeast<br /> | ||
[[1s4e]] - GALK + Mg + ADP + gal - ''Pyrococcus furiosus''<br /> | [[1s4e]] - GALK + Mg + ADP + gal - ''Pyrococcus furiosus''<br /> | ||
Current revision
| |||||||||||
References
- ↑ Holden HM, Thoden JB, Timson DJ, Reece RJ. Galactokinase: structure, function and role in type II galactosemia. Cell Mol Life Sci. 2004 Oct;61(19-20):2471-84. doi: 10.1007/s00018-004-4160-6. PMID:15526155 doi:http://dx.doi.org/10.1007/s00018-004-4160-6
- ↑ Timson DJ, Reece RJ. Functional analysis of disease-causing mutations in human galactokinase. Eur J Biochem. 2003 Apr;270(8):1767-74. PMID:12694189
- ↑ Thoden JB, Timson DJ, Reece RJ, Holden HM. Molecular structure of human galactokinase: implications for type II galactosemia. J Biol Chem. 2005 Mar 11;280(10):9662-70. Epub 2004 Dec 7. PMID:15590630 doi:10.1074/jbc.M412916200
