Galactokinase
From Proteopedia
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| - | <StructureSection load='1wuu' size=' | + | <StructureSection load='1wuu' size='400' side='right' caption='Se-Met human GALK complex with galactopyranose, AMPPNP and Mg+2 ion (green) (PDB code [[1wuu]].' scene='86/864099/Cv/1'> |
__TOC__ | __TOC__ | ||
== Function == | == Function == | ||
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The 3D structure of human GALK shows the sugar binding active site wedged between the N- and C-termini of the enzyme. The galactose forms <scene name='86/864099/Cv/5'>numerous interactions</scene> with GALK termini residues and in <scene name='86/864099/Cv/6'>particular forms hydrogen bonds with an Asp and Arg residues which are conserved in eukaryotic and prokaryotic GALK</scene><ref>PMID:15590630</ref>. | The 3D structure of human GALK shows the sugar binding active site wedged between the N- and C-termini of the enzyme. The galactose forms <scene name='86/864099/Cv/5'>numerous interactions</scene> with GALK termini residues and in <scene name='86/864099/Cv/6'>particular forms hydrogen bonds with an Asp and Arg residues which are conserved in eukaryotic and prokaryotic GALK</scene><ref>PMID:15590630</ref>. | ||
*<scene name='86/864099/Cv/7'>Mg coordination site</scene>. | *<scene name='86/864099/Cv/7'>Mg coordination site</scene>. | ||
| + | *<scene name='86/864099/Cv/9'>AMPPNP binding site</scene>. | ||
| + | *<scene name='86/864099/Cv/10'>Whole binding site</scene>. | ||
==3D structures of galactokinase== | ==3D structures of galactokinase== | ||
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[[6gr2]] - hGALK + ADP + gal - human<br /> | [[6gr2]] - hGALK + ADP + gal - human<br /> | ||
| + | [[7rcl]], [[7rcm]] - hGALK (mutant) + ADP + gal <br /> | ||
[[1wuu]] - hGALK + Mg + AMPPNP + gal<br /> | [[1wuu]] - hGALK + Mg + AMPPNP + gal<br /> | ||
[[6q3w]], [[6q8z]] - hGALK + pyrazine derivative + gal<br /> | [[6q3w]], [[6q8z]] - hGALK + pyrazine derivative + gal<br /> | ||
[[6q91]] - hGALK + benzamide derivative + gal<br /> | [[6q91]] - hGALK + benzamide derivative + gal<br /> | ||
[[6q90]], [[6zgw]] - hGALK + pyridine derivative + gal<br /> | [[6q90]], [[6zgw]] - hGALK + pyridine derivative + gal<br /> | ||
| - | [[6q3x]] - hGALK + quinazoline derivative + gal<br /> | + | [[6q3x]], [[6zfh]], [[7s49]] - hGALK + quinazoline derivative + gal<br /> |
| - | [[6zgv]], [[6zgx]], [[6zgz]], [[6zgy]] - hGALK + pyrimidine derivative + gal<br /> | + | [[6zgv]], [[6zgx]], [[6zgz]], [[6zgy]], [[6zh0]] - hGALK + pyrimidine derivative + gal<br /> |
| + | [[7s4c]] - hGALK (mutant) + pyrimidine derivative + gal<br /> | ||
[[6qje]] - hGALK + imidazole derivative + gal<br /> | [[6qje]] - hGALK + imidazole derivative + gal<br /> | ||
| + | [[7ozx]] - hGALK + inhibitor + gal<br /> | ||
[[2aj4]] - GALK + Mg + AMPPNP + gal - yeast<br /> | [[2aj4]] - GALK + Mg + AMPPNP + gal - yeast<br /> | ||
[[1s4e]] - GALK + Mg + ADP + gal - ''Pyrococcus furiosus''<br /> | [[1s4e]] - GALK + Mg + ADP + gal - ''Pyrococcus furiosus''<br /> | ||
Current revision
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References
- ↑ Holden HM, Thoden JB, Timson DJ, Reece RJ. Galactokinase: structure, function and role in type II galactosemia. Cell Mol Life Sci. 2004 Oct;61(19-20):2471-84. doi: 10.1007/s00018-004-4160-6. PMID:15526155 doi:http://dx.doi.org/10.1007/s00018-004-4160-6
- ↑ Timson DJ, Reece RJ. Functional analysis of disease-causing mutations in human galactokinase. Eur J Biochem. 2003 Apr;270(8):1767-74. PMID:12694189
- ↑ Thoden JB, Timson DJ, Reece RJ, Holden HM. Molecular structure of human galactokinase: implications for type II galactosemia. J Biol Chem. 2005 Mar 11;280(10):9662-70. Epub 2004 Dec 7. PMID:15590630 doi:10.1074/jbc.M412916200
