1k5h
From Proteopedia
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'''1-deoxy-D-xylulose-5-phosphate reductoisomerase''' | '''1-deoxy-D-xylulose-5-phosphate reductoisomerase''' | ||
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[[Category: Stubbs, M T.]] | [[Category: Stubbs, M T.]] | ||
[[Category: Wiesner, J.]] | [[Category: Wiesner, J.]] | ||
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| - | [[Category: | + | [[Category: Beta-barrel]] |
| - | [[Category: | + | [[Category: Dinucleotide binding motif]] |
| - | [[Category: | + | [[Category: Induced fit]] |
| - | [[Category: | + | [[Category: Variable loop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:20:08 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:20, 2 May 2008
1-deoxy-D-xylulose-5-phosphate reductoisomerase
Overview
We have solved the 2.5-A crystal structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, an enzyme involved in the mevalonate-independent 2-C-methyl-D-erythritol-4-phosphate pathway of isoprenoid biosynthesis. The structure reveals that the enzyme is present as a homodimer. Each monomer displays a V-like shape and is composed of an amino-terminal dinucleotide binding domain, a connective domain, and a carboxyl-terminal four-helix bundle domain. The connective domain is responsible for dimerization and harbors most of the active site. The strictly conserved acidic residues Asp(150), Glu(152), Glu(231), and Glu(234) are clustered at the putative active site and are probably involved in the binding of divalent cations mandatory for enzyme activity. The connective and four-helix bundle domains show significant mobility upon superposition of the dinucleotide binding domains of the three conformational states present in the asymmetric unit of the crystal. A still more pronounced flexibility is observed for a loop spanning residues 186 to 216, which adopts two completely different conformations within the three protein conformers. A possible involvement of this loop in an induced fit during substrate binding is discussed.
About this Structure
1K5H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of 1-deoxy-D-xylulose-5-phosphate reductoisomerase, a crucial enzyme in the non-mevalonate pathway of isoprenoid biosynthesis., Reuter K, Sanderbrand S, Jomaa H, Wiesner J, Steinbrecher I, Beck E, Hintz M, Klebe G, Stubbs MT, J Biol Chem. 2002 Feb 15;277(7):5378-84. Epub 2001 Dec 7. PMID:11741911 Page seeded by OCA on Fri May 2 22:20:08 2008
